Rabbit anti-Human RASAL3 Polyclonal Antibody | anti-RASAL3 antibody

RASAL3, CT (RASAL3, RAS protein activator like-3) (MaxLight 490)

Cat. Num. AAA6340422

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: RASAL3; Polyclonal Antibody; CT (RASAL3; RAS protein activator like-3) (MaxLight 490); RAS protein activator like-3; anti-RASAL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RASAL3.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-RASAL3 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 947-975 in the C-terminal region of human RASAL3.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Y79 cell line lysates (35ug/lane), usingMBS649561. This demonstrates that MBS649561 detected the RASAL3 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue, using MBS649561 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS649561 for immunohistochemistry.)

Product Categories/Family for anti-RASAL3 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

1. Kimura, K., et al. Genome Res. 16(1):55-65(2006). 2. Ota, T., et al. Nat. Genet. 36(1):40-45(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 130502162
• NCBI GeneID: 64926
• NCBI Accession #: NP_075055.1
• NCBI GenBank Nucleotide #: NM_022904.1
• UniProt Accession #: Q86YV0
• Molecular Weight: 103 kDa
• NCBI Official Full Name: RAS protein activator like-3 isoform 1
• NCBI Official Synonym Full Names: RAS protein activator like 3
• NCBI Official Symbol: RASAL3
• NCBI Protein Information: RAS protein activator like-3
• UniProt Protein Name: RAS protein activator like-3
• Protein Family: RAS protein activator
• UniProt Gene Name: RASAL3
• UniProt Entry Name: RASL3_HUMAN

NCBI Description

This gene belongs to the Ras GTPase-activating proteins (RasGAP) family and encodes a protein with pleckstrin homology (PH), C2, and Ras GTPase-activation protein (RasGAP) domains. This protein is localized near or at the plasma membrane when expressed exogenously. Reduced expression of this gene in some cell lines resulted in increased levels of the active form of Ras (Ras-GTP), suggesting that this gene may play a role in negatively regulating the Ras signaling pathway. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017]

Uniprot Description

Function: May function as a Ras GTPase-activating protein

By similarity.

Sequence similarities: Contains 1 C2 domain.Contains 1 PH domain.Contains 1 Ras-GAP domain.

Sequence caution: The sequence AAH30281.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB15064.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC56928.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence BAC56928.1 differs from that shown. Reason: Erroneous termination at position 723. Translated as Arg.