Rabbit anti-Human Ptf1a Polyclonal Antibody | anti-Ptf1a antibody

Ptf1a, NT (PTF1A, BHLHA29, PTF1P48, Pancreas transcription factor 1 subunit alpha, Class A basic helix-loop-helix protein 29, Pancreas-specific transcription factor 1a, bHLH transcription factor p48, p48 DNA-binding subunit of transcription factor PTF1) (

Cat. Num. AAA6338690

• Gene Names: PTF1A; bHLHa29; PTF1-p48
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Ptf1a; Polyclonal Antibody; NT (PTF1A; BHLHA29; PTF1P48; Pancreas transcription factor 1 subunit alpha; Class A basic helix-loop-helix protein 29; Pancreas-specific transcription factor 1a; bHLH transcription factor p48; p48 DNA-binding subunit of transcription factor PTF1) (; PTF1A; p48 DNA-binding subunit of transcription factor PTF1; anti-Ptf1a antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-Ptf1a antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: Ptf1a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human Ptf1a.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Ptf1a (arrow) using rabbit polyclonal Ptf1a Antibody (N-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Ptf1a gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with Ptf1a Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-Ptf1a antibody

Ptf1a is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. This protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in the Ptf1a gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers.

Product Categories/Family for anti-Ptf1a antibody

Antibodies; Transcription Factors

References

Yamada, M., J. Neurosci. 27 (41), 10924-10934 (2007). Beres, T.M., Mol. Cell. Biol. 26 (1), 117-130 (2006). Sellick, G.S., Nat. Genet. 36 (12), 1301-1305 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 30039710
• NCBI GeneID: 256297
• NCBI Accession #: NP_835455.1
• NCBI GenBank Nucleotide #: NM_178161.2
• UniProt Accession #: Q7RTS3; Q9HC25
• Molecular Weight: 34,970 Da
• NCBI Official Full Name: pancreas transcription factor 1 subunit alpha
• NCBI Official Synonym Full Names: pancreas specific transcription factor, 1a
• NCBI Official Symbol: PTF1A
• NCBI Official Synonym Symbols: bHLHa29; PTF1-p48
• NCBI Protein Information: pancreas transcription factor 1 subunit alpha; class II bHLH protein PTF1A; bHLH transcription factor p48; class A basic helix-loop-helix protein 29; pancreas-specific transcription factor 1a; exocrine pancreas-specific transcription factor p48; p48 DNA-b
• UniProt Protein Name: Pancreas transcription factor 1 subunit alpha
• Protein Family: Pancreas transcription factor
• UniProt Gene Name: PTF1A
• UniProt Synonym Gene Names: BHLHA29; PTF1P48; bHLHa29; PTF1-p48
• UniProt Entry Name: PTF1A_HUMAN

NCBI Description

This gene encodes a protein that is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers. [provided by RefSeq, Jul 2008]

Uniprot Description

PTF1A: Transcriptional activator. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays an important role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. Defects in PTF1A are the cause of diabetes mellitus and cerebellar hypoplasia/agenesis (DMCH).

Chromosomal Location of Human Ortholog: 10p12.2

Cellular Component: transcription factor complex; cytoplasm; nucleus

Molecular Function: protein dimerization activity; DNA binding; sequence-specific DNA binding; chromatin binding

Biological Process: retinoic acid receptor signaling pathway; tissue development; embryonic development; regulation of transcription, DNA-dependent; transcription, DNA-dependent; pancreas development; exocrine pancreas development; neuron fate commitment; cerebellum development; positive regulation of transcription from RNA polymerase II promoter; endocrine pancreas development

Disease: Pancreatic And Cerebellar Agenesis; Pancreatic Agenesis 2