Rabbit anti-Human PSMD7 Polyclonal Antibody | anti-PSMD7 antibody

PSMD7, CT (PSMD7, MOV34L, 26S proteasome non-ATPase regulatory subunit 7, 26S proteasome regulatory subunit RPN8, 26S proteasome regulatory subunit S12, Mov34 protein homolog, Proteasome subunit p40) (FITC)

Cat. Num. AAA6338318

• Gene Names: PSMD7; P40; S12; Rpn8; MOV34
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PSMD7; Polyclonal Antibody; CT (PSMD7; MOV34L; 26S proteasome non-ATPase regulatory subunit 7; 26S proteasome regulatory subunit RPN8; 26S proteasome regulatory subunit S12; Mov34 protein homolog; Proteasome subunit p40) (FITC); Proteasome subunit p40; anti-PSMD7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PSMD7 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PSMD7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-303 amino acids from the C-terminal region of human PSMD7.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PSMD7 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PSMD7 antibody detected the PSMD7 protein (arrow).)

Immunohistochemistry (IHC)

(PSMD7 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PSMD7 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PSMD7 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PSMD7 antibody

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMD7 is a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 17.

Product Categories/Family for anti-PSMD7 antibody

Antibodies; Proteasomes

References

Dastani, Z., et al. Eur. J. Hum. Genet. 18(3):342-347(2010). Sanches, M., et al. J. Mol. Biol. 370(5):846-855(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 25777615
• NCBI GeneID: 5713
• NCBI Accession #: NP_002802
• NCBI GenBank Nucleotide #: NM_002811
• UniProt Accession #: P51665; Q6PKI2; Q96E97; D3DWS9
• Molecular Weight: 37,025 Da
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 7
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) 26S subunit, non-ATPase, 7
• NCBI Official Symbol: PSMD7
• NCBI Official Synonym Symbols: P40; S12; Rpn8; MOV34
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 7; 26S proteasome regulatory subunit S12; 26S proteasome regulatory subunit rpn8; Moloney leukemia virus-34 proviral integration; Mov34 homolog; proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 7
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD7
• UniProt Synonym Gene Names: MOV34L
• UniProt Entry Name: PSMD7_HUMAN