Rabbit anti-Human, Mouse Prkd3 Polyclonal Antibody | anti-Prkd3 antibody

Prkd3, NT (Prkd3, Prkcn, Serine/threonine-protein kinase D3, Protein kinase C nu type, nPKC-nu) (Azide free) (HRP)

Cat. Num. AAA6337395

• Gene Names: Prkd3; PKD3; Pkcnu; Prkcn; 4930557O20Rik; 5730497N19Rik
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Prkd3; Polyclonal Antibody; NT (Prkd3; Prkcn; Serine/threonine-protein kinase D3; Protein kinase C nu type; nPKC-nu) (Azide free) (HRP); nPKC-nu; anti-Prkd3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Prkd3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: Mouse Prkd3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-249 amino acids from the N-terminal region of human Mouse Prkd3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Prkd3 (arrow) using rabbit polyclonal Mouse Prkd3 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Prkd3 gene.)

Immunohistochemistry (IHC)

(Mouse Prkd3 Antibody (N-term) (AP14141a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse duodenum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Prkd3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-Prkd3 antibody

Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress (By similarity).

Product Categories/Family for anti-Prkd3 antibody

Antibodies; Protein Kinases

References

Chen, L.A., et al. J. Biol. Chem. 284(4):2459-2471(2009). Ellwanger, K., et al. BMC Dev. Biol. 8, 47 (2008): Oster, H., et al. Gene Expr. Patterns 6(4):400-408(2006). Rey, O., et al. J. Biol. Chem. 281(8):5149-5157(2006). Matthews, S.A., et al. J. Biol. Chem. 278(11):9086-9091(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 27753989
• NCBI GeneID: 75292
• NCBI Accession #: NP_083515.2
• NCBI GenBank Nucleotide #: NM_029239.3
• UniProt Accession #: Q8K1Y2
• Molecular Weight: 100,078 Da
• NCBI Official Full Name: serine/threonine-protein kinase D3 isoform 2
• NCBI Official Synonym Full Names: protein kinase D3
• NCBI Official Symbol: Prkd3
• NCBI Official Synonym Symbols: PKD3; Pkcnu; Prkcn; 4930557O20Rik; 5730497N19Rik
• NCBI Protein Information: serine/threonine-protein kinase D3; nPKC-nu; protein kinase C, nu; protein kinase C nu type
• UniProt Protein Name: Serine/threonine-protein kinase D3
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: Prkd3
• UniProt Synonym Gene Names: Prkcn
• UniProt Entry Name: KPCD3_MOUSE

Uniprot Description

PKD3: a CAMK kinase of the PKD family. An important component of signaling pathways downstream from novel PKC enzymes after B-cell receptor engagement. Novel isoforms regulate human keratinocyte differentiation

Protein type: Protein kinase, CAMK; EC 2.7.11.13; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; PKD family

Cellular Component: nucleoplasm; membrane; cytoplasm

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein kinase C activity; metal ion binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: protein amino acid phosphorylation; phosphorylation