Rabbit anti-Human PRELP Polyclonal Antibody | anti-PRELP antibody

PRELP, ID (PRELP, SLRR2A, Prolargin, Proline-arginine-rich end leucine-rich repeat protein) (Azide free) (HRP)

Cat. Num. AAA6337142

• Gene Names: PRELP; MST161; SLRR2A; MSTP161
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: PRELP; Polyclonal Antibody; ID (PRELP; SLRR2A; Prolargin; Proline-arginine-rich end leucine-rich repeat protein) (Azide free) (HRP); Proline-arginine-rich end leucine-rich repeat protein; anti-PRELP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRELP.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PRELP antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 119-148 in the central region of human PRELP. UniProt Accession: P51888.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse heart tissue lysates (15ug/lane)usingMBS647988. This demonstrates that MBS647988 detected PRELP protein (arrow).)

Immunohistochemistry (IHC)

(IHC analysis in formalin fixed and paraffin embedded breast carcinoma using MBS647988 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS647988 for immunohistochemistry.)

Product Categories/Family for anti-PRELP antibody

Antibodies; Leucine-rich Proteins

References

1. Ehret, G.B., et al. Eur. J. Hum. Genet. 17(12):1650-1657(2009). 2. Grover, J., et al. Matrix Biol. 26(2):140-143(2007). 3. Johnson, J.M., et al. Mol. Vis. 12, 1057-1066 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 41349454
• NCBI GeneID: 5549
• NCBI Accession #: NP_958505.1
• NCBI GenBank Nucleotide #: NM_201348.1
• UniProt Accession #: P51888; Q6FG38
• Molecular Weight: 43,810 Da
• NCBI Official Full Name: prolargin
• NCBI Official Synonym Full Names: proline/arginine-rich end leucine-rich repeat protein
• NCBI Official Symbol: PRELP
• NCBI Official Synonym Symbols: MST161; SLRR2A; MSTP161
• NCBI Protein Information: prolargin; prolargin proteoglycan; proline-arginine-rich end leucine-rich repeat protein; 55 kDa leucine-rich repeat protein of articular cartilage
• UniProt Protein Name: Prolargin
• Protein Family: Prolargin
• UniProt Gene Name: PRELP
• UniProt Synonym Gene Names: SLRR2A
• UniProt Entry Name: PRELP_HUMAN

NCBI Description

The protein encoded by this gene is a leucine-rich repeat protein present in connective tissue extracellular matrix. This protein functions as a molecule anchoring basement membranes to the underlying connective tissue. This protein has been shown to bind type I collagen to basement membranes and type II collagen to cartilage. It also binds the basement membrane heparan sulfate proteoglycan perlecan. This protein is suggested to be involved in the pathogenesis of Hutchinson-Gilford progeria (HGP), which is reported to lack the binding of collagen in basement membranes and cartilage. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

PRELP: May anchor basement membranes to the underlying connective tissue. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; lysosomal lumen; Golgi lumen; extracellular region; vesicle

Molecular Function: heparin binding; extracellular matrix structural constituent

Biological Process: keratan sulfate metabolic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; keratan sulfate biosynthetic process; cell aging; pathogenesis; keratan sulfate catabolic process; skeletal development