Rabbit anti-Human PPP1R16B Polyclonal Antibody | anti-PPP1R16B antibody

PPP1R16B, ID (PPP1R16B, ANKRD4, KIAA0823, Protein phosphatase 1 regulatory inhibitor subunit 16B, Ankyrin repeat domain-containing protein 4, CAAX box protein TIMAP, TGF-beta-inhibited membrane-associated protein) (Azide free) (HRP)

Cat. Num. AAA6336504

• Gene Names: PPP1R16B; TIMAP; ANKRD4
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PPP1R16B; Polyclonal Antibody; ID (PPP1R16B; ANKRD4; KIAA0823; Protein phosphatase 1 regulatory inhibitor subunit 16B; Ankyrin repeat domain-containing protein 4; CAAX box protein TIMAP; TGF-beta-inhibited membrane-associated protein) (Azide free) (HRP); TGF-beta-inhibited membrane-associated protein; anti-PPP1R16B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PPP1R16B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PPP1R16B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 371-399 amino acids from the Central region of human PPP1R16B.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PPP1R16B Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PPP1R16B antibody detected the PPP1R16B protein (arrow).)

Related Product Information for anti-PPP1R16B antibody

The protein encoded by this gene is membrane-associated and contains five ankyrin repeats, a protein phosphatase-1-interacting domain, and a carboxy-terminal CAAX box domain. Synthesis of the encoded protein is inhibited by transforming growth factor beta-1. The protein may bind to the membrane through its CAAX box domain and may act as a signaling molecule through interaction with protein phosphatase-1. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.

Product Categories/Family for anti-PPP1R16B antibody

Antibodies; Enzymes; Phosphatase

References

Csortos, C., et al. Am. J. Physiol. Lung Cell Mol. Physiol. 295 (3), L440-L450 (2008): Kim, K., et al. Biochem. Biophys. Res. Commun. 338(3):1327-1334(2005). Homma, K., et al. J. Mol. Biol. 343(5):1207-1220(2004). Cao, W., et al. Am. J. Physiol., Cell Physiol. 283 (1), C327-C337 (2002): Deloukas, P., et al. Nature 414(6866):865-871(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 289629249
• NCBI GeneID: 26051
• NCBI Accession #: NP_001166206.1
• NCBI GenBank Nucleotide #: NM_001172735.1
• UniProt Accession #: Q96T49; O94912; Q9NQG4; A2RRR6
• Molecular Weight: 63,551 Da
• NCBI Official Full Name: protein phosphatase 1 regulatory inhibitor subunit 16B isoform 2
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory subunit 16B
• NCBI Official Symbol: PPP1R16B
• NCBI Official Synonym Symbols: TIMAP; ANKRD4
• NCBI Protein Information: protein phosphatase 1 regulatory inhibitor subunit 16B; hTIMAP; CAAX box protein TIMAP; ankyrin repeat domain protein 4; ankyrin repeat domain-containing protein 4; TGF-beta-inhibited membrane-associated protein; protein phosphatase 1, regulatory (inhibit
• UniProt Protein Name: Protein phosphatase 1 regulatory inhibitor subunit 16B
• Protein Family: Protein phosphatase 1 regulatory inhibitor
• UniProt Gene Name: PPP1R16B
• UniProt Synonym Gene Names: ANKRD4; KIAA0823; hTIMAP
• UniProt Entry Name: PP16B_HUMAN

NCBI Description

The protein encoded by this gene is membrane-associated and contains five ankyrin repeats, a protein phosphatase-1-interacting domain, and a carboxy-terminal CAAX box domain. Synthesis of the encoded protein is inhibited by transforming growth factor beta-1. The protein may bind to the membrane through its CAAX box domain and may act as a signaling molecule through interaction with protein phosphatase-1. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]

Uniprot Description

PPP1R16B: Regulator of protein phosphatase 1 (PP1) that acts as a positive regulator of pulmonary endothelial cell (EC) barrier function. Involved in PKA-mediated moesin dephosphorylation which is important in EC barrier protection against thrombin stimulation. Promotes the interaction of PPP1CA with RPSA/LAMR1 and in turn facilitates the dephosphorylation of RPSA/LAMR1. Involved in the regulation of endothelial cell filopodia extension. May be a downstream target for TGF-beta1 signaling cascade in endothelial cells.

Protein type: Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q11.23

Cellular Component: cell projection; plasma membrane; nucleus

Molecular Function: protein binding; protein phosphatase type 1 regulator activity; protein phosphatase binding

Biological Process: negative regulation of protein amino acid dephosphorylation; regulation of catalytic activity; regulation of phosphoinositide 3-kinase cascade; regulation of filopodium formation; positive regulation of endothelial cell proliferation; signal transduction; positive regulation of protein amino acid dephosphorylation