Rabbit anti-Human PPM1A Polyclonal Antibody | anti-PPM1A antibody

PPM1A (PPM1A, PPPM1A, Protein phosphatase 1A, Protein phosphatase 2C isoform alpha, Protein phosphatase IA) (FITC)

Cat. Num. AAA6336371

• Gene Names: PPM1A; PP2CA; PP2Calpha; PP2C-ALPHA
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PPM1A; Polyclonal Antibody; PPM1A (PPM1A; PPPM1A; Protein phosphatase 1A; Protein phosphatase 2C isoform alpha; Protein phosphatase IA) (FITC); Protein phosphatase IA; anti-PPM1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PPM1A antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PPM1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from human PPM1A.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PPM1A Antibody (pS) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the PPM1A antibody detected the PPM1A protein (arrow).)

Related Product Information for anti-PPM1A antibody

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described.

Product Categories/Family for anti-PPM1A antibody

Antibodies; Enzymes; Phosphatase

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Zhang, B., et al. Histochem. Cell Biol. 132(2):169-179(2009). Sun, W., et al. Cell. Signal. 21(1):95-102(2009). Wang, Y., et al. J. Biol. Chem. 283(48):33578-33584(2008). Bu, S., et al. J. Biol. Chem. 283(28):19593-19602(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 10337595
• NCBI GeneID: 5494
• NCBI Accession #: NP_066283.1; NP_808820.1
• NCBI GenBank Nucleotide #: NM_021003.4
• UniProt Accession #: P35813; O75551; B5BU11; J3KNM0
• Molecular Weight: 382
• NCBI Official Full Name: protein phosphatase 1A isoform 1
• NCBI Official Synonym Full Names: protein phosphatase, Mg2+/Mn2+ dependent, 1A
• NCBI Official Symbol: PPM1A
• NCBI Official Synonym Symbols: PP2CA; PP2Calpha; PP2C-ALPHA
• NCBI Protein Information: protein phosphatase 1A; protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform
• UniProt Protein Name: Protein phosphatase 1A
• Protein Family: Protein phosphatase
• UniProt Gene Name: PPM1A
• UniProt Synonym Gene Names: PPPM1A; PP2C-alpha
• UniProt Entry Name: PPM1A_HUMAN

Uniprot Description

PPPM1A: a Ser/Thr protein phosphatase of the PP2C family. Inhibits the activation of p38 and JNK kinase cascades induced by environmental stresses. Can also dephosphorylate cyclin-dependent kinases and may play a role in cell cycle control. Overexpression of this phosphatase activates the expression of p53, leading to G2/M cell cycle arrest and apoptosis.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16

Chromosomal Location of Human Ortholog: 14q23.1

Cellular Component: nucleoplasm; neuron projection; membrane; voltage-gated calcium channel complex; nucleus; cytosol

Molecular Function: protein C-terminus binding; protein binding; signal transducer activity; calmodulin-dependent protein phosphatase activity; manganese ion binding; magnesium ion binding; protein serine/threonine phosphatase activity

Biological Process: transcription initiation from RNA polymerase II promoter; N-terminal protein myristoylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; Wnt receptor signaling pathway; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; negative regulation of NF-kappaB import into nucleus; negative regulation of I-kappaB kinase/NF-kappaB cascade; protein amino acid dephosphorylation; dephosphorylation; transforming growth factor beta receptor signaling pathway; insulin receptor signaling pathway; gene expression; cell cycle arrest; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of Wnt receptor signaling pathway