Rabbit anti-Human PNPLA3 Polyclonal Antibody | anti-PNPLA3 antibody

PNPLA3, NT (PNPLA3, ADPN, C22orf20, Patatin-like phospholipase domain-containing protein 3, Acylglycerol O-acyltransferase, Adiponutrin, Calcium-independent phospholipase A2-epsilon) (PE)

Cat. Num. AAA6335399

• Gene Names: PNPLA3; ADPN; C22orf20; iPLA(2)epsilon
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: PNPLA3; Polyclonal Antibody; NT (PNPLA3; ADPN; C22orf20; Patatin-like phospholipase domain-containing protein 3; Acylglycerol O-acyltransferase; Adiponutrin; Calcium-independent phospholipase A2-epsilon) (PE); Calcium-independent phospholipase A2-epsilon; anti-PNPLA3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PNPLA3.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PNPLA3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 109-137 in the N-terminal region of human PNPLA3, UniProt Accession #NP_079501.2; Q9NST1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS6005845 in K562 cell line lysates (35ug/lane).This demonstrates that MBS6005845 detected the PNPLA3 protein (arrow).)

Related Product Information for anti-PNPLA3 antibody

The protein encoded by this gene is a triacylglycerol lipase that mediates triacylglycerol hydrolysis in adipocytes. The encoded protein, which appears to be membrane bound, may be involved in the balance of energy usage/storage in adipocytes.

Product Categories/Family for anti-PNPLA3 antibody

Antibodies; Enzymes; Acyltransferase

References

1. Santoro, N., et al. Hepatology 52(4):1281-1290(2010). 2. Valenti, L., et al. Hepatology 52(4):1274-1280(2010). 3. Rotman, Y., et al. Hepatology 52(3):894-903(2010). 4. Speliotes, E.K., et al. Hepatology 52(3):904-912(2010). 5. Pare, G., et al. PLoS Genet. 6 (6), E1000981 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 17196626
• NCBI GeneID: 80339
• NCBI Accession #: NP_079501.2
• NCBI GenBank Nucleotide #: NM_025225.2
• UniProt Accession #: Q9NST1; Q6P1A1; Q96CB4; B0QYI0; B2RCL3; B3KW00
• Molecular Weight: 52,485 Da
• NCBI Official Full Name: patatin-like phospholipase domain-containing protein 3
• NCBI Official Synonym Full Names: patatin-like phospholipase domain containing 3
• NCBI Official Symbol: PNPLA3
• NCBI Official Synonym Symbols: ADPN; C22orf20; iPLA(2)epsilon
• NCBI Protein Information: patatin-like phospholipase domain-containing protein 3; adiponutrin; iPLA2epsilon; iPLA2-epsilon; acylglycerol O-acyltransferase; calcium-independent phospholipase A2-epsilon
• UniProt Protein Name: Patatin-like phospholipase domain-containing protein 3
• Protein Family: Patatin-like phospholipase domain-containing protein
• UniProt Gene Name: PNPLA3
• UniProt Synonym Gene Names: ADPN; C22orf20; iPLA2-epsilon
• UniProt Entry Name: PLPL3_HUMAN

NCBI Description

The protein encoded by this gene is a triacylglycerol lipase that mediates triacylglycerol hydrolysis in adipocytes. The encoded protein, which appears to be membrane bound, may be involved in the balance of energy usage/storage in adipocytes. [provided by RefSeq, Jul 2008]

Uniprot Description

PNPLA3: Multifunctional enzyme which has both triacylglycerol lipase and acylglycerol O-acyltransferase activities. Genetic variations in PNPLA3 are a cause of susceptibility to non-alcoholic fatty liver disease type 1 (NAFLD1). A condition characterized by an accumulation of excess triglyceride in the liver, a condition known as hepatic steatosis (or fatty liver), which is associated with adverse metabolic consequences, including insulin resistance and dyslipidemia. In a subset of individuals, hepatic steatosis promotes an inflammatory response in the liver, referred to as steatohepatitis, which can progress to cirrhosis and liver cancer. NAFLD is the most common form of liver disease in Western countries. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - glycerolipid; Transferase; Lipid Metabolism - glycerophospholipid; Secondary Metabolites Metabolism - limonene and pinene degradation; Phospholipase; Amino Acid Metabolism - tyrosine; Membrane protein, integral; EC 3.1.1.3; Amino Acid Metabolism - phenylalanine

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: endoplasmic reticulum membrane; membrane; integral to membrane

Molecular Function: phospholipase A2 activity; triacylglycerol lipase activity; mono-olein transacylation activity; diolein transacylation activity

Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; triacylglycerol catabolic process; triacylglycerol biosynthetic process