Western Blot (WB)
(Western blot analysis of PIK3R2 (arrow) using rabbit polyclonal PIK3R2 Antibody (T447) (RB13726). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIK3R2 gene (Lane 2) (Origene Technologies).)
Rabbit anti-Human PIK3R2 Polyclonal Antibody | anti-PIK3R2 antibody
PIK3R2 (Y464) (PIK3R2, Phosphatidylinositol 3-kinase regulatory subunit beta, Phosphatidylinositol 3-kinase 85kD regulatory subunit beta) (FITC)
Gene Names
PIK3R2; p85; MPPH; P85B; p85-BETA
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PIK3R2; Polyclonal Antibody; PIK3R2 (Y464) (PIK3R2; Phosphatidylinositol 3-kinase regulatory subunit beta; Phosphatidylinositol 3-kinase 85kD regulatory subunit beta) (FITC); Phosphatidylinositol 3-kinase 85kD regulatory subunit beta; anti-PIK3R2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-PIK3R2 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:50-100
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
PIK3R2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y464 of human PIK3R2.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of PIK3R2 (arrow) using rabbit polyclonal PIK3R2 Antibody (T447) (RB13726). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIK3R2 gene (Lane 2) (Origene Technologies).)
Western Blot (WB)
(Western blot analysis of PIK3R2 (arrow) using rabbit polyclonal PIK3R2 Antibody (T447) (RB13726). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIK3R2 gene (Lane 2) (Origene Technologies).)
Related Product Information for anti-PIK3R2 antibody
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The family has been classified into 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. PIK3R2 binds to activated Protein Tyrosine Kinases, which are phosphorylated, through its SH2 domain, and acts as an adaptor, mediating the association of the P110 catalytic unit to the plasma membrane.
Product Categories/Family for anti-PIK3R2 antibody
References
Khan, N.A., et al., J. Neurovirol. 9(6):584-593 (2003). Deregibus, M.C., et al., J. Biol. Chem. 277(28):25195-25202 (2002). Cook, J.A., et al., J. Immunol. 169(1):254-260 (2002). Park, I.W., et al., Blood 97(2):352-358 (2001). Zauli, G., et al., FASEB J. 15(2):483-491 (2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
728
NCBI Official Full Name
phosphatidylinositol 3-kinase regulatory subunit beta
NCBI Official Synonym Full Names
phosphoinositide-3-kinase, regulatory subunit 2 (beta)
NCBI Official Symbol
PIK3R2
NCBI Official Synonym Symbols
p85; MPPH; P85B; p85-BETA
NCBI Protein Information
phosphatidylinositol 3-kinase regulatory subunit beta; PI3-kinase subunit p85-beta; PI3K regulatory subunit beta; ptdIns-3-kinase regulatory subunit p85-beta; phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; phosphatidylinositol 3-kinase, reg
UniProt Protein Name
Phosphatidylinositol 3-kinase regulatory subunit beta
Protein Family
UniProt Gene Name
PIK3R2
UniProt Synonym Gene Names
PI3-kinase regulatory subunit beta; PI3K regulatory subunit beta; PtdIns-3-kinase regulatory subunit beta; PI3-kinase subunit p85-beta
UniProt Entry Name
P85B_HUMAN
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Product Notes
The PIK3R2 pik3r2 (Catalog #AAA6334050) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIK3R2 (Y464) (PIK3R2, Phosphatidylinositol 3-kinase regulatory subunit beta, Phosphatidylinositol 3-kinase 85kD regulatory subunit beta) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PIK3R2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:50-100 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PIK3R2 pik3r2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIK3R2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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