Rabbit anti-Human PIGA Polyclonal Antibody | anti-PIGA antibody

PIGA, CT (PIGA, Phosphatidylinositol N-acetylglucosaminyltransferase subunit A, GlcNAc-PI synthesis protein, Phosphatidylinositol-glycan biosynthesis class A protein) (Azide free) (HRP)

Cat. Num. AAA6333809

• Gene Names: PIGA; GPI3; PNH1; PIG-A; MCAHS2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PIGA; Polyclonal Antibody; CT (PIGA; Phosphatidylinositol N-acetylglucosaminyltransferase subunit A; GlcNAc-PI synthesis protein; Phosphatidylinositol-glycan biosynthesis class A protein) (Azide free) (HRP); Phosphatidylinositol-glycan biosynthesis class A protein; anti-PIGA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PIGA antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PIGA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-177 amino acids from the C-terminal region of human PIGA.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PIGA Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the PIGA antibody detected the PIGA protein (arrow).)

Immunohistochemistry (IHC)

(PIGA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIGA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PIGA antibody

This gene encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized. A related pseudogene is located on chromosome 12.

Product Categories/Family for anti-PIGA antibody

Antibodies; Enzymes; Glycosyltransferase

References

Borowitz, M.J., et al. Cytometry B Clin Cytom 78(4):211-230(2010). Peruzzi, B., et al. Mutat. Res. 705(1):3-10(2010). Araten, D.J., et al. Mutat. Res. 686 (1-2), 1-8 (2010): Iida, Y., et al. Blood 83(11):3126-3131(1994) Ware, R.E., et al. Blood 83(9):2418-2422(1994).

NCBI and Uniprot Product Information

• NCBI GI #: 299782546
• NCBI GeneID: 5277
• NCBI Accession #: NP_065206.3; NP_002632.1
• NCBI GenBank Nucleotide #: NM_020473.3
• UniProt Accession #: P37287; Q16025; Q16250; B4E0V2
• Molecular Weight: 28,034 Da
• NCBI Official Full Name: phosphatidylinositol N-acetylglucosaminyltransferase subunit A isoform 3
• NCBI Official Synonym Full Names: phosphatidylinositol glycan anchor biosynthesis class A
• NCBI Official Symbol: PIGA
• NCBI Official Synonym Symbols: GPI3; PNH1; PIG-A; MCAHS2
• NCBI Protein Information: phosphatidylinositol N-acetylglucosaminyltransferase subunit A
• UniProt Protein Name: Phosphatidylinositol N-acetylglucosaminyltransferase subunit A
• Protein Family: Phosphatidylinositol N-acetylglucosaminyltransferase
• UniProt Gene Name: PIGA
• UniProt Synonym Gene Names: PIG-A
• UniProt Entry Name: PIGA_HUMAN

NCBI Description

This gene encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized. A related pseudogene is located on chromosome 12. [provided by RefSeq, Jun 2010]