Rabbit anti-Human PDLIM1 Polyclonal Antibody | anti-PDLIM1 antibody

PDLIM1, CT (PDLIM1, CLIM1, CLP36, PDZ and LIM domain protein 1, C-terminal LIM domain protein 1, Elfin, LIM domain protein CLP-36) (Azide free) (HRP)

Cat. Num. AAA6332478

• Gene Names: PDLIM1; CLIM1; CLP36; CLP-36; hCLIM1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PDLIM1; Polyclonal Antibody; CT (PDLIM1; CLIM1; CLP36; PDZ and LIM domain protein 1; C-terminal LIM domain protein 1; Elfin; LIM domain protein CLP-36) (Azide free) (HRP); LIM domain protein CLP-36; anti-PDLIM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PDLIM1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PDLIM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-282 amino acids from the C-terminal region of human PDLIM1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PDLIM1 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PDLIM1 antibody detected the PDLIM1 protein (arrow).)

Related Product Information for anti-PDLIM1 antibody

PDLIM1 is a Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton.

Product Categories/Family for anti-PDLIM1 antibody

Antibodies; PDZ Domain

References

Chapuis, J., et al. Mol. Psychiatry 14(11):1004-1016(2009). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Silverberg, M.S., et al. Eur. J. Hum. Genet. 15(3):328-335(2007). Miehe, U., et al. Histochem. Cell Biol. 126(4):465-471(2006). Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 13994151
• NCBI GeneID: 9124
• NCBI Accession #: NP_066272.1
• NCBI GenBank Nucleotide #: NM_020992.3
• UniProt Accession #: O00151; Q5VZH5; Q9BPZ9; B2RBS6
• Molecular Weight: 36,072 Da
• NCBI Official Full Name: PDZ and LIM domain protein 1
• NCBI Official Synonym Full Names: PDZ and LIM domain 1
• NCBI Official Symbol: PDLIM1
• NCBI Official Synonym Symbols: CLIM1; CLP36; CLP-36; hCLIM1
• NCBI Protein Information: PDZ and LIM domain protein 1; elfin; LIM domain protein CLP-36; carboxyl terminal LIM domain protein 1
• UniProt Protein Name: PDZ and LIM domain protein 1
• Protein Family: PDZ and LIM domain protein
• UniProt Gene Name: PDLIM1
• UniProt Synonym Gene Names: CLIM1; CLP36
• UniProt Entry Name: PDLI1_HUMAN

NCBI Description

This gene encodes a member of the enigma protein family. The protein contains two protein interacting domains, a PDZ domain at the amino terminal end and one to three LIM domains at the carboxyl terminal. It is a cytoplasmic protein associated with the cytoskeleton. The protein may function as an adapter to bring other LIM-interacting proteins to the cytoskeleton. Pseudogenes associated with this gene are located on chromosomes 3, 14 and 17. [provided by RefSeq, Oct 2012]

Uniprot Description

CLIM1: a cytoskeletal adaptor protein that bring other proteins to the cytoskeleton. Interacts with alpha-actinins 1, 2 and 4. Recruits the Clik1 kinase to actin stress fibers in nonmuscle cells. Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 10q23.1

Cellular Component: transcription factor complex; cytoskeleton; focal adhesion; cytoplasm

Molecular Function: zinc ion binding; transcription coactivator activity

Biological Process: regulation of transcription, DNA-dependent; response to hypoxia; response to oxidative stress