Rabbit anti-Human PDF Polyclonal Antibody | anti-PDF antibody

PDF, ID (PDF, PDF1A, Peptide deformylase, mitochondrial, Polypeptide deformylase) (FITC)

Cat. Num. AAA6332246

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PDF; Polyclonal Antibody; ID (PDF; PDF1A; Peptide deformylase; mitochondrial; Polypeptide deformylase) (FITC); Polypeptide deformylase; anti-PDF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PDF antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PDF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-151 amino acids from the Central region of human PDF.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PDF Antibody (Center) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the PDF antibody detected the PDF protein (arrow).)

Related Product Information for anti-PDF antibody

Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells.

Product Categories/Family for anti-PDF antibody

Antibodies; Mitochondrial Proteins

References

Escobar-Alvarez, S., et al. J. Mol. Biol. 387(5):1211-1228(2009). Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3558-3566(2008). Lee, M.D., et al. J. Clin. Invest. 114(8):1107-1116(2004). Serero, A., et al. J. Biol. Chem. 278(52):52953-52963(2003). Lee, M.D., et al. Biochem. Biophys. Res. Commun. 312(2):309-315(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 11641243
• NCBI GeneID: 64146
• NCBI Accession #: NP_071736.1
• NCBI GenBank Nucleotide #: NM_022341.1
• UniProt Accession #: Q9HBH1; Q8WUN6
• Molecular Weight: 27,013 Da
• NCBI Official Full Name: peptide deformylase, mitochondrial
• NCBI Official Synonym Full Names: peptide deformylase (mitochondrial)
• NCBI Official Symbol: PDF
• NCBI Protein Information: peptide deformylase, mitochondrial; polypeptide deformylase; peptide deformylase-like protein
• UniProt Protein Name: Peptide deformylase, mitochondrial
• Protein Family: Protein
• UniProt Gene Name: PDF
• UniProt Synonym Gene Names: PDF1A
• UniProt Entry Name: DEFM_HUMAN

Uniprot Description

PDF: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Belongs to the polypeptide deformylase family.

Protein type: EC 3.5.1.88; Hydrolase; Mitochondrial

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: mitochondrion

Molecular Function: iron ion binding; peptide deformylase activity

Biological Process: translation; peptidyl-methionine modification; positive regulation of cell proliferation; N-terminal protein amino acid modification