Mouse Acetylcholinesterase YT blood group Monoclonal Antibody | anti-ACHE antibody

Acetylcholinesterase YT blood group (Acetylcholine acetylhydrolase, ACHE, precursor, ARACHE, N-ACHE-YT)

Cat. Num. AAA632930

• Gene Names: ACHE; YT; ACEE; ARACHE; N-ACHE
• Reactivity: Human, Monkey
• Applications: ELISA
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Acetylcholinesterase YT blood group; Monoclonal Antibody; Acetylcholinesterase YT blood group (Acetylcholine acetylhydrolase; ACHE; precursor; ARACHE; N-ACHE-YT); Anti -Acetylcholinesterase YT blood group (Acetylcholine acetylhydrolase; anti-ACHE antibody

• Host: Mouse
• Reactivity: Human, Monkey
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2Q6
• Specificity: Recognizes human Acetylcholinesterase
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.

• Applicable Applications for anti-ACHE antibody: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA.
• Immunogen: Acetylcholinesterase AChE from human erythrocyte.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-ACHE antibody

Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.

Product Categories/Family for anti-ACHE antibody

Antibodies; Abs to Enzymes; Acetylcholinesterase

NCBI and Uniprot Product Information

• NCBI GI #: 4557239
• NCBI GeneID: 43
• NCBI Accession #: NP_000656.1
• NCBI GenBank Nucleotide #: NM_000665.3
• UniProt Accession #: P22303; Q16169; Q29S23; Q2M324; Q504V3; Q53F46; Q86TM9; Q86YX9; Q9BXP7; A4D2E2; B7ZKZ0; D6W5X7
• Molecular Weight: 67,796 Da
• NCBI Official Full Name: acetylcholinesterase isoform E4-E6
• NCBI Official Synonym Full Names: acetylcholinesterase
• NCBI Official Symbol: ACHE
• NCBI Official Synonym Symbols: YT; ACEE; ARACHE; N-ACHE
• NCBI Protein Information: acetylcholinesterase; Yt blood group; apoptosis-related acetylcholinesterase
• UniProt Protein Name: Acetylcholinesterase
• UniProt Gene Name: ACHE
• UniProt Synonym Gene Names: AChE
• UniProt Entry Name: ACES_HUMAN

NCBI Description

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]

Uniprot Description

ACHE: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. Belongs to the type-B carboxylesterase/lipase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - glycerophospholipid; EC 3.1.1.7; Hydrolase; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: Golgi apparatus; extracellular space; cell surface; membrane; perinuclear region of cytoplasm; plasma membrane; extracellular region; basal lamina; synapse; cell junction; neuromuscular junction; nucleus

Molecular Function: collagen binding; protein binding; serine hydrolase activity; protein homodimerization activity; cholinesterase activity; protein self-association; hydrolase activity; beta-amyloid binding; laminin binding; acetylcholinesterase activity; acetylcholine binding

Biological Process: nervous system development; negative regulation of synaptic transmission, cholinergic; muscle development; acetylcholine catabolic process in synaptic cleft; acetylcholine catabolic process; glycerophospholipid biosynthetic process; osteoblast development; cell proliferation; synaptic transmission; synaptogenesis; positive regulation of protein secretion; retina development in camera-type eye; amyloid precursor protein metabolic process; phospholipid metabolic process; neurotransmitter receptor biosynthetic process; receptor internalization; response to wounding; phosphatidylcholine biosynthetic process; regulation of receptor recycling; DNA replication; cell adhesion; protein tetramerization; neurotransmitter biosynthetic process

Disease: Yt Blood Group Antigen