Rabbit Natriuretic Peptide Receptor A Polyclonal Antibody | anti-ANPRA antibody

Natriuretic Peptide Receptor A, NT (NPR1, ANPRA, Atrial natriuretic peptide receptor 1, Atrial natriuretic peptide receptor type A, Guanylate cyclase A) (AP)

Cat. Num. AAA6323968

• Gene Names: NPR1; ANPa; NPRA; ANPRA; GUC2A; GUCY2A
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Natriuretic Peptide Receptor A; Polyclonal Antibody; NT (NPR1; ANPRA; Atrial natriuretic peptide receptor 1; Atrial natriuretic peptide receptor type A; Guanylate cyclase A) (AP); NPR1; Guanylate cyclase A; anti-ANPRA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-ANPRA antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Natriuretic Peptide Receptor A Antibody antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human ANPA.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Natriuretic Peptide Receptor A (NPR1/ANPA) antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-ANPRA antibody

Antibodies; Receptors

References

Takahashi, Y., et al., Biochem. Biophys. Res. Commun. 246(3):736-739 (1998). Pardhasaradhi, K., et al., Cell. Mol. Neurobiol. 14(1):1-7 (1994). Lowe, D.G., et al., EMBO J. 8(5):1377-1384 (1989).

NCBI and Uniprot Product Information

• NCBI GI #: 167830411
• NCBI GeneID: 4881
• NCBI Accession #: NP_000897.3
• NCBI GenBank Nucleotide #: NM_000906.3
• UniProt Accession #: P16066; Q5SR08; Q6P4Q3; B0ZBF0
• Molecular Weight: 118,919 Da
• NCBI Official Full Name: atrial natriuretic peptide receptor 1
• NCBI Official Synonym Full Names: natriuretic peptide receptor 1
• NCBI Official Symbol: NPR1
• NCBI Official Synonym Symbols: ANPa; NPRA; ANPRA; GUC2A; GUCY2A
• NCBI Protein Information: atrial natriuretic peptide receptor 1
• UniProt Protein Name: Atrial natriuretic peptide receptor 1
• UniProt Gene Name: NPR1
• UniProt Synonym Gene Names: ANPRA; ANP-A; ANPR-A; NPR-A; GC-A

NCBI Description

Guanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms (Garbers and Lowe, 1994 [PubMed 7982997]). The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A (NPR1) and type B (NPR2; MIM 108961). Also see NPR3 (MIM 108962), which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides (ANP (MIM 108780) and BNP (MIM 600295), respectively).[supplied by OMIM, May 2009]

Uniprot Description

Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.