Mouse Transcription Factor YY1 Monoclonal Antibody | anti-Yy1 antibody

Transcription Factor YY1 (DELTA, NF-E1, UCRBP, Yin Yang 1)

Cat. Num. AAA632300

• Gene Names: Yy1; NF-E1; UCRBP
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Gel Super Shift Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Transcription Factor YY1; Monoclonal Antibody; Transcription Factor YY1 (DELTA; NF-E1; UCRBP; Yin Yang 1); Anti -Transcription Factor YY1 (DELTA; anti-Yy1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 8J147
• Specificity: Recognizes human Transcription Factor YY1.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 1% BSA and 0.05% sodium azide.

• Applicable Applications for anti-Yy1 antibody: Western Blot (WB), Gel Shift Assay (GS/EMSA)
• Application Notes: Suitable for use in Dot Blot and Western Blot.
• Immunogen: Partial sequence of recombinant full-length protein to human Transcription Factor YY1
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

Testing Data

Related Product Information for anti-Yy1 antibody

The ubiquitous transcription factor Yin Yang 1 (YY1) is known to have a fundamental role in normal biologic processes such as embryogenesis, differentiation, replication, and cellular proliferation. YY1 exerts its effects on genes involved in these processes via its ability to initiate, activate, or repress transcription depending upon the context in which it binds. Mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. YY1 activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression; however, these mechanisms have not yet been fully elucidated. Since expression and function of YY1 are known to be intimately associated with progression through phases of the cell cycle, the physiologic significance of YY1 activity has recently been applied to models of tumor biology. The majority of the data are consistent with the hypothesis that YY1 overexpression and/or activation is associated with unchecked cellular proliferation, resistance to apoptotic stimuli, tumorigenesis and metastatic potential. Studies involving hematopoetic tumors, epithelial-based tumors, endocrine organ malignancies, hepatocellular carcinoma, and retinoblastoma support this hypothesis. Molecular mechanisms that have been investigated include YY1-mediated downregulation of p53 activity, interference with poly-ADP-ribose polymerase, alteration in c-myc and nuclear factor-kappa B (NF-kappaB) expression, regulation of death genes and gene products, and differential YY1 binding in the presence of inflammatory mediators. Further, recent findings implicate YY1 in the regulation of tumor cell resistance to chemotherapeutics and immune-mediated apoptotic stimuli. Taken together, these findings provide strong support of the hypothesis that YY1, in addition to its regulatory roles in normal biologic processes, may possess the potential to act as an initiator of tumorigenesis and may thus serve as both a prognostic tumor marker; furthermore, it may provide an effective target for antitumor chemotherapy and/or immunotherapy.

Product Categories/Family for anti-Yy1 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 38231568
• NCBI GeneID: 24919
• UniProt Accession #: Q8CHJ4
• Molecular Weight: 44,417 Da
• NCBI Official Full Name: transcription factor YY1
• NCBI Official Synonym Full Names: YY1 transcription factor
• NCBI Official Symbol: Yy1
• NCBI Official Synonym Symbols: NF-E1; UCRBP
• NCBI Protein Information: transcriptional repressor protein YY1
• UniProt Protein Name: Transcription factor YY1
• Protein Family: Transcriptional repressor protein
• UniProt Gene Name: Yy1
• UniProt Synonym Gene Names: yy1
• UniProt Entry Name: Q8CHJ4_RAT

NCBI Description

acts as a transcriptional repressor of the steroidogenic acute regulatory (StaR) protein; decreases SREBP-1a binding to a sterol regulatory element [RGD]

Uniprot Description

YY1: a polycomb-group (Pc-G) protein essential for early mammalian development and the regulation of X-inactivation and certain imprinted genes. Required for the transactivation of TSIX by CTCF. The complex of CTCF-YY1-TSIX is a critical regulator of the X chromosome binary switch. Can function as an activator or a repressor depending on the presence of other proteins. For example it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence.

Protein type: Transcription factor; C2H2-type zinc finger protein; Cell development/differentiation

Cellular Component: transcription factor complex; nuclear matrix; cytoplasm; PcG protein complex; nucleus

Molecular Function: protein binding; DNA binding; four-way junction DNA binding; sequence-specific DNA binding; RNA binding; histone deacetylase binding; metal ion binding; SMAD binding; transcription factor activity

Biological Process: anterior/posterior pattern formation; transcription from RNA polymerase II promoter; RNA localization; camera-type eye morphogenesis; regulation of transcription, DNA-dependent; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus; chromosome organization and biogenesis; double-strand break repair via homologous recombination; response to UV-C