Rabbit anti-Human, Mouse MSR1 Polyclonal Antibody | anti-MSR1 antibody

MSR1, CT (MSR1, SCARA1, Macrophage scavenger receptor types I and II, Macrophage acetylated LDL receptor I and II, Scavenger receptor class A member 1, CD204) (Azide free) (HRP)

Cat. Num. AAA6322564

• Gene Names: MSR1; SRA; SR-A; CD204; phSR1; phSR2; SCARA1
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: MSR1; Polyclonal Antibody; CT (MSR1; SCARA1; Macrophage scavenger receptor types I and II; Macrophage acetylated LDL receptor I and II; Scavenger receptor class A member 1; CD204) (Azide free) (HRP); CD204; anti-MSR1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MSR1. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MSR1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 336-364 in the C-terminal region of human MSR1, UniProt Accession #NP_619729; P21757. Species sequence homology: bovine
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane) usingMBS6001549.)

Related Product Information for anti-MSR1 antibody

This gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages.

Product Categories/Family for anti-MSR1 antibody

Antibodies; Receptors; Scavenger

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Wang, Y., et al. J. Hum. Genet. 55(8):490-494(2010). Voruganti, V.S., et al. Am. J. Clin. Nutr. 91(6):1574-1583(2010). Nonomura, N., et al. Cancer Sci. 101(6):1570-1573(2010). Seizer, P., et al. Semin. Thromb. Hemost. 36(2):157-162(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 20357512
• NCBI GeneID: 4481
• NCBI Accession #: NP_619729.1; NP_619730.1
• NCBI GenBank Nucleotide #: NM_138715.2
• UniProt Accession #: P21757; O60505; P21759; Q45F10; D3DSP3
• Molecular Weight: 49,762 Da
• NCBI Official Full Name: macrophage scavenger receptor types I and II isoform type 1
• NCBI Official Synonym Full Names: macrophage scavenger receptor 1
• NCBI Official Symbol: MSR1
• NCBI Official Synonym Symbols: SRA; SR-A; CD204; phSR1; phSR2; SCARA1
• NCBI Protein Information: macrophage scavenger receptor types I and II; scavenger receptor class A member 1; scavenger receptor class A, member 1; macrophage scavenger receptor type III; macrophage acetylated LDL receptor I and II
• UniProt Protein Name: Macrophage scavenger receptor types I and II
• Protein Family: Macrophage scavenger receptor
• UniProt Gene Name: MSR1
• UniProt Synonym Gene Names: SCARA1
• UniProt Entry Name: MSRE_HUMAN

NCBI Description

This gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages. [provided by RefSeq, Jul 2008]

Uniprot Description

MSR1: Membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL). Isoform III does not internalize actetylated LDL. Defects in MSR1 may be a cause of prostate cancer (PC). A malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet- ring cell carcinoma and neuroendocrine carcinoma. MSR1 variants may play a role in susceptibility to prostate cancer. MSR1 variants have been found in individuals with prostate cancer and co-segregate with the disease in some families. Defects in MSR1 may be a cause of Barrett esophagus (BE). A condition characterized by a metaplastic change in which normal esophageal squamous epithelium is replaced by a columnar and intestinal-type epithelium. Patients with Barrett esophagus have an increased risk of esophageal adenocarcinoma. The main cause of Barrett esophagus is gastroesophageal reflux. The retrograde movement of acid and bile salts from the stomach into the esophagus causes prolonged injury to the esophageal epithelium and induces chronic esophagitis, which in turn is believed to trigger the pathologic changes. Genetic variants in MSR1 have been found in individuals with Barrett esophagus and are thought to contribute to disease susceptibility. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: collagen; integral to plasma membrane; plasma membrane; cytosol

Molecular Function: protein binding; low-density lipoprotein binding; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; cholesterol transport; lipoprotein transport

Disease: Prostate Cancer; Barrett Esophagus