Rabbit anti-Human MMP13 Polyclonal Antibody | anti-MMP13 antibody

MMP13, ID (MMP13, Collagenase 3, Matrix metalloproteinase-13) (Biotin)

Cat. Num. AAA6321330

• Gene Names: MMP13; CLG3; MANDP1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: MMP13; Polyclonal Antibody; ID (MMP13; Collagenase 3; Matrix metalloproteinase-13) (Biotin); Matrix metalloproteinase-13; anti-MMP13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MMP13.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MMP13 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 253-282 in the central region of human MMP13, Swiss/UniProt Accession: P45452. Species sequence homology: equine and rabbit
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS647316. This demonstrates that MBS647316 detected the MMP13 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma using MBS647316 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS647316 for immunohistochemistry.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis with Hela cells using MBS647316 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-MMP13 antibody

Antibodies; Matrix Metalloproteinases

References

1. Miranda, K.J., et al. J. Biol. Chem. 285(41):31517-31524 (2010). 2. Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010). 3. Nikopensius, T., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(9):748-756 (2010). 4. Yamada, T., et al. Anticancer Res. 30(7):2693-2699(2010). 5. Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4505209
• NCBI GeneID: 4322
• NCBI Accession #: NP_002418.1
• NCBI GenBank Nucleotide #: NM_002427.3
• UniProt Accession #: P45452
• Molecular Weight: 53,820 Da
• NCBI Official Full Name: collagenase 3 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 13 (collagenase 3)
• NCBI Official Symbol: MMP13
• NCBI Official Synonym Symbols: CLG3; MANDP1
• NCBI Protein Information: collagenase 3; MMP-13; matrix metalloproteinase 13 (collagenase 3)
• UniProt Protein Name: Collagenase 3
• Protein Family: Collagenase
• UniProt Gene Name: MMP13
• UniProt Synonym Gene Names: MMP-13
• UniProt Entry Name: MMP13_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

Uniprot Description

MMP13: Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process. Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO). A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age. Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1). Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia. Belongs to the peptidase M10A family.

Protein type: Secreted; Secreted, signal peptide; EC 3.4.24.-; Protease

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: collagen binding; zinc ion binding; metalloendopeptidase activity; calcium ion binding

Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; cellular protein metabolic process; proteolysis; bone mineralization; endochondral ossification

Disease: Spondyloepimetaphyseal Dysplasia, Missouri Type