Rabbit anti-Human MLNR Polyclonal Antibody | anti-MLNR antibody

MLNR, CT (MLNR, GPR38, MTLR, MTLR1, Motilin receptor, G-protein coupled receptor 38) (Biotin)

Cat. Num. AAA6321187

• Gene Names: MLNR; GPR38; MTLR1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MLNR; Polyclonal Antibody; CT (MLNR; GPR38; MTLR; MTLR1; Motilin receptor; G-protein coupled receptor 38) (Biotin); G-protein coupled receptor 38; anti-MLNR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MLNR.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MLNR antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 357-386 in the C-terminal region of human MLNR, UniProt Accession #O43193.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H292 cell line lysates using MBS6002303 (35ug/lane). This demonstrates the MLNR antibody detected the MLNR protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue using MBS6002303 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Product Categories/Family for anti-MLNR antibody

Antibodies; G Protein Receptors

References

1. Yokoyama, K., et al. Nephron Clin Pract 115 (4), C237-C243 (2010). 2. Mitselos, A., et al. Peptides 29(7):1167-1175(2008). 3. Ter Beek, W.P., et al. Inflamm. Bowel Dis. 14(5):612-619(2008). 4. Tokunaga, H., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 294 (2), G460-G466 (2008). 5. Dowal, L., et al. J. Biol. Chem. 281(33):23999-24014(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 4504095
• NCBI GeneID: 2862
• NCBI Accession #: NP_001498.1
• NCBI GenBank Nucleotide #: NM_001507.1
• UniProt Accession #: O43193
• Molecular Weight: 45,344 Da
• NCBI Official Full Name: motilin receptor
• NCBI Official Synonym Full Names: motilin receptor
• NCBI Official Symbol: MLNR
• NCBI Official Synonym Symbols: GPR38; MTLR1
• NCBI Protein Information: motilin receptor; G protein-coupled receptor 38
• UniProt Protein Name: Motilin receptor
• Protein Family: Motilin receptor
• UniProt Gene Name: MLNR
• UniProt Synonym Gene Names: GPR38; MTLR; MTLR1
• UniProt Entry Name: MTLR_HUMAN

NCBI Description

Motilin is a 22 amino acid peptide hormone expressed throughout the gastrointestinal (GI) tract. The protein encoded by this gene is a motilin receptor which is a member of the G-protein coupled receptor 1 family. This member is a multi-pass transmembrane protein, and is an important therapeutic target for the treatment of hypomotility disorders. [provided by RefSeq, Aug 2011]

Uniprot Description

MLNR: Receptor for motilin. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 13q14-q21

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; growth hormone-releasing hormone receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; digestion