Western Blot (WB)
(METTL11B Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the METTL11B antibody detected the METTL11B protein (arrow).)
Rabbit anti-Mouse METTL11B Polyclonal Antibody | anti-METTL11B antibody
METTL11B, NT (METTL11B, C1orf184, Alpha N-terminal protein methyltransferase 1B, Methyltransferase-like protein 11B, X-Pro-Lys N-terminal protein methyltransferase 1B) (APC)
Gene Names
METTL11B; NTM1B; HOMT1B; C1orf184
Reactivity
Mouse
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
METTL11B; Polyclonal Antibody; NT (METTL11B; C1orf184; Alpha N-terminal protein methyltransferase 1B; Methyltransferase-like protein 11B; X-Pro-Lys N-terminal protein methyltransferase 1B) (APC); X-Pro-Lys N-terminal protein methyltransferase 1B; anti-METTL11B antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-METTL11B antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
METTL11B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human METTL11B.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(METTL11B Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the METTL11B antibody detected the METTL11B protein (arrow).)
Western Blot (WB)
(METTL11B Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the METTL11B antibody detected the METTL11B protein (arrow).)
Related Product Information for anti-METTL11B antibody
METTL11B is alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di-or tri-methylation of exposed alpha-amino group of Ala, Pro or Ser residue in the [Ala/Pro/Ser]-Pro-Lys motif (By similarity).
Product Categories/Family for anti-METTL11B antibody
References
Gregory, S.G., et al. Nature 441(7091):315-321(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,400 Da
NCBI Official Full Name
alpha N-terminal protein methyltransferase 1B
NCBI Official Synonym Full Names
methyltransferase like 11B
NCBI Official Symbol
METTL11B
NCBI Official Synonym Symbols
NTM1B; HOMT1B; C1orf184
NCBI Protein Information
alpha N-terminal protein methyltransferase 1B; methyltransferase-like protein 11B; X-Pro-Lys N-terminal protein methyltransferase 1B
UniProt Protein Name
Alpha N-terminal protein methyltransferase 1B
Protein Family
UniProt Gene Name
METTL11B
UniProt Synonym Gene Names
C1orf184; NTM1B
UniProt Entry Name
NTM1B_HUMAN
Uniprot Description
Function: Alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif
By similarity.
Catalytic activity: 3 S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] = 3 S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein].2 S-adenosyl-L-methionine + N-terminal-PPK-[protein] = 2 S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein].
Sequence similarities: Belongs to the methyltransferase superfamily. NTM1 family.
Sequence caution: The sequence CAH72139.1 differs from that shown. Reason: Erroneous gene model prediction.
Similar Products
Product Notes
The METTL11B mettl11b (Catalog #AAA6320394) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The METTL11B, NT (METTL11B, C1orf184, Alpha N-terminal protein methyltransferase 1B, Methyltransferase-like protein 11B, X-Pro-Lys N-terminal protein methyltransferase 1B) (APC) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's METTL11B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the METTL11B mettl11b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "METTL11B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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