Rabbit anti-Human MEIS2 Polyclonal Antibody | anti-MEIS2 antibody

MEIS2, ID (D269) (MEIS2, MRG1, Homeobox protein Meis2, Meis1-related protein 1) (APC)

Cat. Num. AAA6320167

• Gene Names: MEIS2; MRG1; CPCMR; HsT18361
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MEIS2; Polyclonal Antibody; ID (D269) (MEIS2; MRG1; Homeobox protein Meis2; Meis1-related protein 1) (APC); Meis1-related protein 1; anti-MEIS2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-MEIS2 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MEIS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 261-290 amino acids from the Central region of human MEIS2.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MEIS2 Antibody (Center D269) western blot analysis in WiDr cell line lysates (15ug/lane).This demonstrates the MEIS2 antibody detected the MEIS2 protein (arrow).)

Immunohistochemistry (IHC)

(MEIS2 Antibody (Center D269) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MEIS2 Antibody (Center D269) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(MEIS2 Antibody (Center D269) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MEIS2 antibody

MEIS2 is a homeobox protein belonging to the TALE ('three amino acid loop extension') family of homeodomain-containing proteins. TALE homeobox proteins are highly conserved transcription regulators, and several members have been shown to be essential contributors to developmental programs.

Product Categories/Family for anti-MEIS2 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

Adkins, D.E., et al. Mol. Psychiatry (2010). Milech, N., et al. Leuk. Res. 34(3):358-363(2010). Vasan, R.S., et al. JAMA 302(2):168-178(2009). Steelman, S., et al. Genome Res. 7(2):142-156(1997).

NCBI and Uniprot Product Information

• NCBI GI #: 24762241
• NCBI GeneID: 4212
• NCBI Accession #: NP_733775
• NCBI GenBank Nucleotide #: NM_170675
• UniProt Accession #: O14770
• Molecular Weight: 52kDa
• NCBI Official Full Name: homeobox protein Meis2 isoform c
• NCBI Official Synonym Full Names: Meis homeobox 2
• NCBI Official Symbol: MEIS2
• NCBI Official Synonym Symbols: MRG1; CPCMR; HsT18361
• NCBI Protein Information: homeobox protein Meis2
• UniProt Protein Name: Homeobox protein Meis2
• Protein Family: Homeobox protein
• UniProt Gene Name: MEIS2
• UniProt Synonym Gene Names: MRG1
• UniProt Entry Name: MEIS2_HUMAN

NCBI Description

This gene encodes a homeobox protein belonging to the TALE ('three amino acid loop extension') family of homeodomain-containing proteins. TALE homeobox proteins are highly conserved transcription regulators, and several members have been shown to be essential contributors to developmental programs. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MEIS2: a homeobox protein belonging to the TALE ('three amino acid loop extension') family of homeodomain-containing proteins. TALE homeobox proteins are highly conserved transcription regulators, and several members have been shown to be essential contributors to developmental programs. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: nucleus

Molecular Function: protein binding; sequence-specific DNA binding; transcription cofactor activity; transcription factor activity; transcription factor binding; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; negative regulation of myeloid cell differentiation; eye development; response to mechanical stimulus; pancreas development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter