Rabbit anti-Human MCOLN1 Polyclonal Antibody | anti-MCOLN1 antibody

MCOLN1, CT (MCOLN1, ML4, Mucolipin-1, MG-2, Mucolipidin) (FITC)

Cat. Num. AAA6319741

• Gene Names: MCOLN1; ML1; ML4; MG-2; MLIV; MST080; TRPML1; MSTP080; TRP-ML1; TRPM-L1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: MCOLN1; Polyclonal Antibody; CT (MCOLN1; ML4; Mucolipin-1; MG-2; Mucolipidin) (FITC); Mucolipidin; anti-MCOLN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MCOLN1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-MCOLN1 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 531-560 in the C-terminal region of human MCOLN1, UniProt Accession: #Q9GZU1. Species sequence homology: monkey, mouse
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of Jurkat cell line lysates (35ug/lane) usingMBS6007836. This demonstrates that MBS6007836 detected the MCOLN1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using MBS6007836 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6007836 for immunohistochemistry.)

Product Categories/Family for anti-MCOLN1 antibody

Antibodies; Proteins

References

1. Eichelsdoerfer, J.L., et al. J. Biol. Chem. 285(45):34304-34308 (2010). 2. Curcio-Morelli, C., et al. J. Cell. Physiol. 222(2):328-335 (2010). 3. Vergarajauregui, S., et al. J. Biol. Chem. 284(52):36357-36366 (2009). 4. Ballif, B.A., et al. Mol. Cell Proteomics 3(11):1093-1101 (2004). 5. LaPlante, J.M., et al. FEBS Lett. 532 (1-2), 183-187 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 10092597
• NCBI GeneID: 57192
• NCBI Accession #: NP_065394.1
• NCBI GenBank Nucleotide #: NM_020533.2
• UniProt Accession #: Q9GZU1; Q7Z4F7; Q9H292; Q9H4B3; Q9H4B5; D6W647
• Molecular Weight: 65,022 Da
• NCBI Official Full Name: mucolipin-1
• NCBI Official Synonym Full Names: mucolipin 1
• NCBI Official Symbol: MCOLN1
• NCBI Official Synonym Symbols: ML1; ML4; MG-2; MLIV; MST080; TRPML1; MSTP080; TRP-ML1; TRPM-L1
• NCBI Protein Information: mucolipin-1
• UniProt Protein Name: Mucolipin-1
• Protein Family: Mucolipin
• UniProt Gene Name: MCOLN1
• UniProt Synonym Gene Names: ML4; ML1; TRPML11 PublicationManual assertion based on opinion iniRef.28

NCBI Description

This gene encodes a memberof the transient receptor potential (TRP) cation channel gene family. The transmembrane protein localizes to intracellular vesicular membranes including lysosomes, and functions in the late endocytic pathway and in the regulation of lysosomal exocytosis. The channel is permeable to Ca(2+), Fe(2+), Na(+), K(+), and H(+), and is modulated by changes in Ca(2+) concentration. Mutations in this gene result in mucolipidosis type IV. [provided by RefSeq, Oct 2009]

Uniprot Description

Nonselective cation channel probably playing a role in the regulation of membrane trafficking events and of metal homeostasis. Proposed to play a major role in Ca2+ release from late endosome and lysosome vesicles to the cytoplasm, which is important for many lysosome-dependent cellular events, including the fusion and trafficking of these organelles, exocytosis and autophagy (PubMed:11013137, PubMed:12459486, PubMed:15336987, PubMed:14749347, PubMed:29019983). Required for efficient uptake of large particles in macrophages in which Ca2+ release from the lysosomes triggers lysosomal exocytosis. May also play a role in phagosome-lysosome fusion (). Involved in lactosylceramide trafficking indicative for a role in the regulation of late endocytic membrane fusion/fission events (PubMed:16978393). By mediating lysosomal Ca2+ release is involved in regulation of mTORC1 signaling and in mTOR/TFEB-dependent lysosomal adaptation to environmental cues such as nutrient levels (PubMed:27787197, PubMed:25733853). Seems to act as lysosomal active oxygen species (ROS) sensor involved in ROS-induced TFEB activation and autophagy (PubMed:27357649). Functions as a Fe2+ permeable channel in late endosomes and lysosomes (PubMed:18794901). Proposed to play a role in zinc homeostasis probably implicating its association with TMEM163 (PubMed:25130899) In adaptive immunity, TRPML2 and TRPML1 may play redundant roles in the function of the specialized lysosomes of B cells ().