Rabbit MCL1 Polyclonal Antibody | anti-MCL1 antibody

MCL1, BH3 Domain Specific (MCL1, BCL2L3, Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, mcl1/EAT) (Biotin)

Cat. Num. AAA6319641

• Gene Names: MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: MCL1; Polyclonal Antibody; BH3 Domain Specific (MCL1; BCL2L3; Induced myeloid leukemia cell differentiation protein Mcl-1; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; mcl1/EAT) (Biotin); mcl1/EAT; anti-MCL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the BH3 domain of human MCL1 at ~37kD. Species Crossreactivity: mouse, rat
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MCL1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 191-226 in the BH3 domain of human MCL1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Ramos cell line lysates (35ug/lane) usingMBS6001583. Mcl-1-BH3(arrow) was detected usingMBS6001583.)

Testing Data

(All lanes: MBS6001583 (1:2000) Lane 1: Raji whole cell lysate Lane 2: Ramos whole cell lysate Lane 3: A431 whole cell lysate Lane 4: F9 whole cell lysate Lane 5: A20 whole cell lysate. Lysates at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 37kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human breast carcinoma with MBS6001583 which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS6001583 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of ZR-75-1 cells using MBS6001583 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-MCL1 antibody

Antibodies; Cancer Markers; Leukemia

References

1. Crossley, L.J., J. Leukoc. Biol. 74(4):583-592 (2003). 2. Kotelkin, A., et al., J. Virol. 77(17):9156-9172 (2003). 3. Erwert, R.D., et al., Microb. Pathog. 35(2):87-93 (2003). 4. Liu, H., et al., Blood 102(1):344-352 (2003). 5. Nijhawan, D., et al., Genes Dev. 17(12):1475-1486 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 309747067
• NCBI GeneID: 4170
• NCBI Accession #: NP_001184249.1
• NCBI GenBank Nucleotide #: NM_001197320.1
• UniProt Accession #: Q07820; Q9HD91; Q9NRQ3; Q9NRQ4; Q9UHR7; Q9UHR8; Q9UHR9; Q9UNJ1; B2R6B2; D3DV03; D3DV04
• Molecular Weight: 37,337 Da
• NCBI Official Full Name: induced myeloid leukemia cell differentiation protein Mcl-1 isoform 3
• NCBI Official Synonym Full Names: myeloid cell leukemia sequence 1 (BCL2-related)
• NCBI Official Symbol: MCL1
• NCBI Official Synonym Symbols: TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
• NCBI Protein Information: induced myeloid leukemia cell differentiation protein Mcl-1; bcl-2-like protein 3; myeloid cell leukemia ES; bcl-2-related protein EAT/mcl1
• UniProt Protein Name: Induced myeloid leukemia cell differentiation protein Mcl-1
• Protein Family: Minichromosome loss protein
• UniProt Gene Name: MCL1
• UniProt Synonym Gene Names: BCL2L3; Bcl2-L-3
• UniProt Entry Name: MCL1_HUMAN

NCBI Description

This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]

Uniprot Description

MCL1: a myeloid cell leukemia protein of the Bcl-2 family of proteins. Two alternatively spliced transcripts encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.

Protein type: Membrane protein, integral; Mitochondrial; Channel, misc.; Inhibitor; Apoptosis

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; mitochondrial outer membrane; mitochondrion; membrane; mitochondrial matrix; cytoplasm; integral to membrane; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; protein channel activity; BH3 domain binding

Biological Process: apoptotic mitochondrial changes; DNA damage response, signal transduction resulting in induction of apoptosis; response to cytokine stimulus; multicellular organismal development; cellular homeostasis; cell fate determination