Rabbit anti-Human MBL2 Polyclonal Antibody | anti-MBL2 antibody

MBL2, CT (MBL2, COLEC1, MBL, Mannose-binding protein C, Collectin-1, MBP1, Mannan-binding protein, Mannose-binding lectin) (FITC)

Cat. Num. AAA6319367

• Gene Names: MBL2; MBL; MBP; MBP1; MBPD; MBL2D; MBP-C; COLEC1; HSMBPC
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MBL2; Polyclonal Antibody; CT (MBL2; COLEC1; MBL; Mannose-binding protein C; Collectin-1; MBP1; Mannan-binding protein; Mannose-binding lectin) (FITC); Mannose-binding lectin; anti-MBL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MBL2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-MBL2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 173-202 in the C-terminal region of human MBL2, UniProt Accession #P11226.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS6000174 in A549 cell line lysates (35ug/lane). This demonstrates the MBL2 antibody detected the MBL2 protein (arrow).)

Western Blot (WB)

(Western Blot analysis of MBL2 (arrow) usingMBS6000174. 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MBL2 gene.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of MBS6000174 with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of MBS6000174 in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Related Product Information for anti-MBL2 antibody

This gene encodes the soluble mannose-binding lectin or mannose-binding protein found in serum. The protein encoded belongs to the collectin family and is an important element in the innate immune system. The protein recognizes mannose and N-acetylglucosamine on many microorganisms, and is capable of activating the classical complement pathway. Deficiencies of this gene have been associated with susceptibility to autoimmune and infectious diseases.

Product Categories/Family for anti-MBL2 antibody

Antibodies; Lectins

References

1. Auriti, C., et al. Hum. Immunol. 71(11):1084-1088(2010). 2. Fraser, D.A., et al. J. Immunol. 185(7):3932-3939(2010). 3. de Wit, E., et al. Mamm. Genome (2010). 4. Filho, R.M., et al. Viral Immunol. 23(4):449-453(2010). 5. Hu, Y., et al. Viral Immunol. 23(4):443-447(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4557739
• NCBI GeneID: 4153
• NCBI Accession #: NP_000233.1
• NCBI GenBank Nucleotide #: NM_000242.2
• UniProt Accession #: P11226; Q4VB12; Q4VB13; Q86SI4; Q96KE4; Q96TF7; Q96TF8; Q96TF9
• Molecular Weight: 26,144 Da
• NCBI Official Full Name: mannose-binding protein C
• NCBI Official Synonym Full Names: mannose-binding lectin (protein C) 2, soluble
• NCBI Official Symbol: MBL2
• NCBI Official Synonym Symbols: MBL; MBP; MBP1; MBPD; MBL2D; MBP-C; COLEC1; HSMBPC
• NCBI Protein Information: mannose-binding protein C; collectin-1; mannan-binding lectin; mannose-binding lectin 2, soluble (opsonic defect); mannose-binding lectin (protein C) 2, soluble (opsonic defect)
• UniProt Protein Name: Mannose-binding protein C
• Protein Family: Mannose-binding protein
• UniProt Gene Name: MBL2
• UniProt Synonym Gene Names: COLEC1; MBL; MBP-C
• UniProt Entry Name: MBL2_HUMAN

NCBI Description

This gene encodes the soluble mannose-binding lectin or mannose-binding protein found in serum. The protein encoded belongs to the collectin family and is an important element in the innate immune system. The protein recognizes mannose and N-acetylglucosamine on many microorganisms, and is capable of activating the classical complement pathway. Deficiencies of this gene have been associated with susceptibility to autoimmune and infectious diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

MBL2: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; cell surface; collagen; extracellular region

Molecular Function: mannose binding; protein binding; calcium ion binding; calcium-dependent protein binding; receptor binding

Biological Process: defense response to Gram-positive bacterium; killing by host of symbiont cells; positive regulation of phagocytosis; defense response to bacterium; acute-phase response; innate immune response; response to oxidative stress; opsonization; complement activation, lectin pathway; complement activation, classical pathway; negative regulation of viral reproduction; complement activation

Disease: Mannose-binding Protein Deficiency