Rabbit anti-Human MBD3L1 Polyclonal Antibody | anti-MBD3L1 antibody

MBD3L1, CT (MBD3L1, MBD3L, Methyl-CpG-binding domain protein 3-like 1) (Azide free) (HRP)

Cat. Num. AAA6319280

• Gene Names: MBD3L1; MBD3L
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MBD3L1; Polyclonal Antibody; CT (MBD3L1; MBD3L; Methyl-CpG-binding domain protein 3-like 1) (Azide free) (HRP); Methyl-CpG-binding domain protein 3-like 1; anti-MBD3L1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MBD3L1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MBD3L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 167-194 amino acids from the C-terminal region of human MBD3L1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MBD3L1 Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the MBD3L1 antibody detected the MBD3L1 protein (arrow).)

Related Product Information for anti-MBD3L1 antibody

This gene encodes a protein that is related to methyl-CpG-binding proteins but lacks the methyl-CpG binding domain. The protein is localized to discrete areas in the nucleus, and expression appears to be restricted to round spermatids, suggesting that the protein plays a role in the postmeiotic stages of male germ cell development.

Product Categories/Family for anti-MBD3L1 antibody

Antibodies; Binding Proteins

References

Jiang, C.L., et al. J. Biol. Chem. 279(50):52456-52464(2004). Yao, Y.L., et al. J. Biol. Chem. 278(43):42560-42568(2003). Jiang, C.L., et al. Genomics 80(6):621-629(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 226502212
• NCBI GeneID: 85509
• NCBI Accession #: NP_660209.2
• NCBI GenBank Nucleotide #: NM_145208.2
• UniProt Accession #: Q8WWY6; Q2M291; B5BUM6
• Molecular Weight: 21,616 Da
• NCBI Official Full Name: methyl-CpG-binding domain protein 3-like 1
• NCBI Official Synonym Full Names: methyl-CpG binding domain protein 3-like 1
• NCBI Official Symbol: MBD3L1
• NCBI Official Synonym Symbols: MBD3L
• NCBI Protein Information: methyl-CpG-binding domain protein 3-like 1; MBD3-like protein 1
• UniProt Protein Name: Methyl-CpG-binding domain protein 3-like 1
• Protein Family: Methyl-CpG-binding domain protein
• UniProt Gene Name: MBD3L1
• UniProt Synonym Gene Names: MBD3L; MBD3-like protein 1
• UniProt Entry Name: MB3L1_HUMAN

NCBI Description

This gene encodes a protein that is related to methyl-CpG-binding proteins but lacks the methyl-CpG binding domain. The protein is localized to discrete areas in the nucleus, and expression appears to be restricted to round spermatids, suggesting that the protein plays a role in the postmeiotic stages of male germ cell development. [provided by RefSeq, Jul 2008]

Uniprot Description

MBD3L1: Transcriptional repressor. Belongs to the MBD3L family.

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; cytoplasm; plasma membrane

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent