Related Product Information for anti-MBD3 antibody
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex.
Product Categories/Family for anti-MBD3 antibody
References
Bachmann, N., et al. Eur J Med Genet 53(1):23-24(2010). Noh, E.J., et al. Biochem. Biophys. Res. Commun. 378(3):332-337(2009). Morey, L., et al. Mol. Cell. Biol. 28(19):5912-5923(2008). Brown, S.E., et al. Gene 420(2):99-106(2008). Spensberger, D., et al. Biochemistry 47(24):6418-6426(2008).
NCBI Description
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. [provided by RefSeq, Jul 2008]
Uniprot Description
MBD3: methyl-CpG binding (MBD) proteins appear to bind differentially methylated domains (DMDs) of DNA, to recruit repression complexes, and to silence the locus. The methyl CpG binding domain of human MBD3 may not directly bind mCpG but does interact with the NuRD/Mi2 components HDAC1 and MTA2. Plays a role in histone deacetylation and nucleosome remodeling. Increased levels have been reported in lung cancer and glioblastomas and may be a therapeutic target.
Protein type: Gene silencing; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: nucleoplasm; heterochromatin; protein complex; nuclear chromatin; cytoplasm; NuRD complex
Molecular Function: protein binding; nucleosomal DNA binding; DNA binding; methyl-CpG binding
Biological Process: tissue development; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; methylation-dependent chromatin silencing; in utero embryonic development; ATP-dependent chromatin remodeling; negative regulation of transcription from RNA polymerase II promoter; histone acetylation
Product Notes
The MBD3 mbd3 (Catalog #
AAA6319266) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MBD3, NT (MBD3, Methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MBD3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MBD3 mbd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
It is sometimes possible for the material contained within the vial of
"MBD3, Polyclonal Antibody" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
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purchaser may be intending to use a product outside of our accepted criteria.
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