Western Blot (WB)
(Mouse Map3k1 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Map3k1 antibody detected the Map3k1 protein (arrow).)
Rabbit anti-Mouse Map3k1 Polyclonal Antibody | anti-Map3k1 antibody
Map3k1, CT (Map3k1, Mekk, Mekk1, Mitogen-activated protein kinase kinase kinase 1, MAPK/ERK kinase kinase 1) (APC)
Gene Names
Map3k1; Mekk; MEKK1; MAPKKK1
Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Map3k1; Polyclonal Antibody; CT (Map3k1; Mekk; Mekk1; Mitogen-activated protein kinase kinase kinase 1; MAPK/ERK kinase kinase 1) (APC); MAPK/ERK kinase kinase 1; anti-Map3k1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-Map3k1 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
Mouse Map3k1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1193-1221 amino acids from the C-terminal region of Mouse Map3k1.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Mouse Map3k1 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Map3k1 antibody detected the Map3k1 protein (arrow).)
Western Blot (WB)
(Mouse Map3k1 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Map3k1 antibody detected the Map3k1 protein (arrow).)
Immunohistochemistry (IHC)
(Mouse Map3k1 Antibody (C-term) (AP14625b)immunohistochemistry analysis in formalin fixed and paraffin embedded m.heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map3k1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Mouse Map3k1 Antibody (C-term) (AP14625b)immunohistochemistry analysis in formalin fixed and paraffin embedded m.heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map3k1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-Map3k1 antibody
Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.
Product Categories/Family for anti-Map3k1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
161,289 Da
NCBI Official Full Name
Mitogen-activated protein kinase kinase kinase 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 1
NCBI Official Symbol
Map3k1
NCBI Official Synonym Symbols
Mekk; MEKK1; MAPKKK1
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 1; MEKK 1; MEK kinase 1; MAPK/ERK kinase kinase 1; mitogen activated protein kinase kinase kinase 1
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 1
Protein Family
UniProt Gene Name
Map3k1
UniProt Synonym Gene Names
Mekk; Mekk1; MEK kinase 1; MEKK 1
UniProt Entry Name
M3K1_MOUSE
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Product Notes
The Map3k1 map3k1 (Catalog #AAA6318540) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Map3k1, CT (Map3k1, Mekk, Mekk1, Mitogen-activated protein kinase kinase kinase 1, MAPK/ERK kinase kinase 1) (APC) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Map3k1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Map3k1 map3k1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Map3k1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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