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Western Blot (WB)
(Western Blot analysis in 293 cell lysates (2ug/lane) usingMBS646290. Lane 1: Nontransfected Lane 2: Transiently transfected with the AOF2 gene.)
Rabbit anti-Human LSD1 Polyclonal Antibody | anti-LSD1 antibody
LSD1, CT (KDM1A, AOF2, KDM1, KIAA0601, LSD1, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2) (APC)
Gene Names
KDM1A; AOF2; KDM1; LSD1; BHC110
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Immunofluorescence, ELISA
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
LSD1; Polyclonal Antibody; CT (KDM1A; AOF2; KDM1; KIAA0601; Lysine-specific histone demethylase 1A; BRAF35-HDAC complex protein BHC110; Flavin-containing amine oxidase domain-containing protein 2) (APC); KDM1A; Flavin-containing amine oxidase domain-containing protein 2; anti-LSD1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human LSD1.
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-LSD1 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 819-852 in the C-terminal region of human LSD1, UniProt Accession #O60341. Species sequence homology: Mouse
Conjugate
APC
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot analysis in 293 cell lysates (2ug/lane) usingMBS646290. Lane 1: Nontransfected Lane 2: Transiently transfected with the AOF2 gene.)
Western Blot (WB)
(Western Blot analysis in 293 cell lysates (2ug/lane) usingMBS646290. Lane 1: Nontransfected Lane 2: Transiently transfected with the AOF2 gene.)
Western Blot (WB)
(Western Blot of nuclear extracts of control and PhIP-treated HMEC. Proteins were transferred onto polyvinylidene difluoride and blotted withMBS646290. Nuclear LSD1 protein levels increased in carcinogen-treated HMEC compared with control HMEC.)
Western Blot (WB)
(Western Blot of nuclear extracts of control and PhIP-treated HMEC. Proteins were transferred onto polyvinylidene difluoride and blotted withMBS646290. Nuclear LSD1 protein levels increased in carcinogen-treated HMEC compared with control HMEC.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human cancer tissue using MBS646290 followed by peroxidase-conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS646290 for immunohistochemistry)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human cancer tissue using MBS646290 followed by peroxidase-conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS646290 for immunohistochemistry)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of 293 cells using MBS646290 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of 293 cells using MBS646290 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)
Product Categories/Family for anti-LSD1 antibody
References
1. Forneris, F., et al. FEBS Lett. 579 (10), 2203-2207 (2005). 2. Shi, Y., et al. Cell 119 (7), 941-953 (2004). 3. Hakimi, M.A., et al. J. Biol. Chem. 278 (9), 7234-7239 (2003). 4. Hakimi, M.A., et al. PNAS 99 (11), 7420-7425 (2002). 5. Humphrey, G.W., et al. J. Biol. Chem. 276 (9), 6817-6824 (2001). 6. Ota, T., et al., Nat. Genet. 36(1):40-45 (2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
852
NCBI Official Full Name
lysine-specific histone demethylase 1A isoform a
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 1A
NCBI Official Symbol
KDM1A
NCBI Official Synonym Symbols
AOF2; KDM1; LSD1; BHC110
NCBI Protein Information
lysine-specific histone demethylase 1A; lysine (K)-specific demethylase 1; BRAF35-HDAC complex protein BHC110; amine oxidase (flavin containing) domain 2; FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit; flavin-containing amine oxidase domain-con
UniProt Protein Name
Lysine-specific histone demethylase 1A
Protein Family
UniProt Gene Name
KDM1A
UniProt Synonym Gene Names
AOF2; KDM1; KIAA0601; LSD1
UniProt Entry Name
KDM1A_HUMAN
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Product Notes
The LSD1 kdm1a (Catalog #AAA6317451) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LSD1, CT (KDM1A, AOF2, KDM1, KIAA0601, LSD1, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LSD1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunofluorescence (IF), ELISA (EIA). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the LSD1 kdm1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LSD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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