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Western Blot (WB) (Western Blot analysis in HL-60 cell line lysates (35ug/lane) usingMBS6006976. LRG1 (arrow) was detected using the purified Pab.)

Rabbit anti-Human LRG1 Polyclonal Antibody | anti-LRG1 antibody

LRG1, NT (LRG1, LRG, Leucine-rich alpha-2-glycoprotein) (MaxLight 750)

Gene Names
LRG1; LRG; HMFT1766
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
LRG1; Polyclonal Antibody; NT (LRG1; LRG; Leucine-rich alpha-2-glycoprotein) (MaxLight 750); Leucine-rich alpha-2-glycoprotein; anti-LRG1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human LRG1.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Applicable Applications for anti-LRG1 antibody
Flow Cytometry (FC/FACS), Western Blot (WB), FLISA, Immunohistochemistry (IHC) Paraffin
Application Notes
FC/FACS: 1:10-1:50
WB: 1:1000
IHC-P: 1:50-1:100
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 86-114 in the N-terminal region of human LRG1, UniProt Accession #P02750.
Conjugate
MaxLight750
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HL-60 cell line lysates (35ug/lane) usingMBS6006976. LRG1 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western Blot analysis in HL-60 cell line lysates (35ug/lane) usingMBS6006976. LRG1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western Blot analysis in 293 cell lysates (2ug/lane) usingMBS6006976, either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LRG1 gene.)

Western Blot (WB) (Western Blot analysis in 293 cell lysates (2ug/lane) usingMBS6006976, either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LRG1 gene.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded lung carcinoma using MBS6006976 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6006976 for immunohistochemistry.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis in formalin fixed and paraffin embedded lung carcinoma using MBS6006976 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6006976 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometry analysis of HL-60 cells using MBS6006976 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow Cytometry analysis of HL-60 cells using MBS6006976 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-LRG1 antibody
References
1. Shirai, R., Biochem. Biophys. Res. Commun. 382 (4), 776-779 (2009). 2. Gillott, D.J., Fertil. Steril. 90 (3), 761-768 (2008).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,178 Da
NCBI Official Full Name
leucine-rich alpha-2-glycoprotein
NCBI Official Synonym Full Names
leucine-rich alpha-2-glycoprotein 1
NCBI Official Symbol
LRG1
NCBI Official Synonym Symbols
LRG; HMFT1766
NCBI Protein Information
leucine-rich alpha-2-glycoprotein; 1300008B03Rik; 2310031E04Rik; leucine rich alpha 2 glycoprotein
UniProt Protein Name
Leucine-rich alpha-2-glycoprotein
UniProt Gene Name
LRG1
UniProt Synonym Gene Names
LRG; LRG
UniProt Entry Name
A2GL_HUMAN

NCBI Description

The leucine-rich repeat (LRR) family of proteins, including LRG1, have been shown to be involved in protein-protein interaction, signal transduction, and cell adhesion and development. LRG1 is expressed during granulocyte differentiation (O'Donnell et al., 2002 [PubMed 12223515]).[supplied by OMIM, Mar 2008]

Uniprot Description

LRG1:

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: transforming growth factor beta receptor binding

Biological Process: positive regulation of angiogenesis; positive regulation of transforming growth factor beta receptor signaling pathway; brown fat cell differentiation; positive regulation of endothelial cell proliferation

Research Articles on LRG1

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Product Notes

The LRG1 lrg1 (Catalog #AAA6316898) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LRG1, NT (LRG1, LRG, Leucine-rich alpha-2-glycoprotein) (MaxLight 750) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LRG1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB), FLISA, Immunohistochemistry (IHC) Paraffin. FC/FACS: 1:10-1:50 WB: 1:1000 IHC-P: 1:50-1:100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the LRG1 lrg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LRG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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