Rabbit anti-Human LNPEP Polyclonal Antibody | anti-LNPEP antibody

LNPEP, NT (LNPEP, OTASE, Leucyl-cystinyl aminopeptidase, Insulin-regulated membrane aminopeptidase, Insulin-responsive aminopeptidase, Oxytocinase, Placental leucine aminopeptidase, Leucyl-cystinyl aminopeptidase, pregnancy serum form) (MaxLight 750)

Cat. Num. AAA6316634

• Gene Names: LNPEP; CAP; IRAP; PLAP; P-LAP
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: LNPEP; Polyclonal Antibody; NT (LNPEP; OTASE; Leucyl-cystinyl aminopeptidase; Insulin-regulated membrane aminopeptidase; Insulin-responsive aminopeptidase; Oxytocinase; Placental leucine aminopeptidase; pregnancy serum form) (MaxLight 750); pregnancy serum form; anti-LNPEP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LNPEP.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-LNPEP antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 23-52 in the N-terminal region of human LNPEP. Species sequence homology: mouse, rat
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HL-60 cell line lysates (35ug/lane) usingMBS644388. This demonstrates that 037841detected the LNPEP protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue using MBS644388 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS644388 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HL-60 cells (right histogram) using MBS644388 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-LNPEP antibody

Antibodies; Enzymes; Protease (Proteinase; Peptidase)

References

1. Georgiadou, D., et al. J. Immunol. 185(3):1584-1592(2010). 2. Williams, P.J., et al. Placenta 31(5):448-455(2010). 3. Landau, R., et al. Neuro Endocrinol. Lett. 31(1):63-66(2010). 4. Johnson, M.P., et al. Hum. Genet. 126(5):655-666(2009). 5. Saveanu, L., et al. Science 325(5937):213-217(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 61742777
• NCBI GeneID: 4012
• NCBI Accession #: NP_005566.2; NP_787116.2
• NCBI GenBank Nucleotide #: NM_005575.2
• UniProt Accession #: Q9UIQ6; O00769; Q15145; Q59H76; Q9TNQ2; Q9TNQ3; Q9UIQ7
• Molecular Weight: 117,349 Da
• NCBI Official Full Name: leucyl-cystinyl aminopeptidase isoform 1
• NCBI Official Synonym Full Names: leucyl/cystinyl aminopeptidase
• NCBI Official Symbol: LNPEP
• NCBI Official Synonym Symbols: CAP; IRAP; PLAP; P-LAP
• NCBI Protein Information: leucyl-cystinyl aminopeptidase; otase; oxytocinase; vasopressinase; AT (4) receptor; angiotensin IV receptor; insulin-regulated aminopeptidase; placental leucine aminopeptidase; insulin-responsive aminopeptidase; insulin-regulated membrane aminopeptidase
• UniProt Protein Name: Leucyl-cystinyl aminopeptidase
• Protein Family: Leucyl-cystinyl aminopeptidase
• UniProt Gene Name: LNPEP
• UniProt Synonym Gene Names: OTASE; Cystinyl aminopeptidase; IRAP; OTase; P-LAP
• UniProt Entry Name: LCAP_HUMAN

NCBI Description

This gene encodes a zinc-dependent aminopeptidase that cleaves vasopressin, oxytocin, lys-bradykinin, met-enkephalin, dynorphin A and other peptide hormones. The protein can be secreted in maternal serum, reside in intracellular vesicles with the insulin-responsive glucose transporter GLUT4, or form a type II integral membrane glycoprotein. The protein catalyzes the final step in the conversion of angiotensinogen to angiotensin IV (AT4) and is also a receptor for AT4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

IRAP: a leucyl/cystinyl aminopeptidase. A type II membrane protein and secreted. Resides in intracellular vesicles together with GLUT4 and can then translocate to the cell surface in response to insulin and/or oxytocin. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain. Three alternatively spliced isoforms have been described.

Protein type: EC 3.4.11.3; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q15

Cellular Component: cytoplasmic vesicle membrane; membrane; perinuclear region of cytoplasm; lysosomal membrane; integral to plasma membrane; extracellular region; plasma membrane; intracellular; cytosol

Molecular Function: zinc ion binding; metallopeptidase activity; aminopeptidase activity

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; protein polyubiquitination; cell-cell signaling; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I; protein catabolic process; female pregnancy; proteolysis