Western Blot (WB)
(LCE2B Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the LCE2B antibody detected the LCE2B protein (arrow).)
Rabbit anti-Human LCE2B Polyclonal Antibody | anti-LCE2B antibody
LCE2B, CT (LCE2B, LEP10, SPRL1B, XP5, Late cornified envelope protein 2B, Late envelope protein 10, Skin-specific protein Xp5, Small proline-rich-like epidermal differentiation complex protein 1B) (Azide free) (HRP)
Gene Names
LCE2B; XP5; LEP10; SPRL1B
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
LCE2B; Polyclonal Antibody; CT (LCE2B; LEP10; SPRL1B; XP5; Late cornified envelope protein 2B; Late envelope protein 10; Skin-specific protein Xp5; Small proline-rich-like epidermal differentiation complex protein 1B) (Azide free) (HRP); Small proline-rich-like epidermal differentiation complex protein 1B; anti-LCE2B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-LCE2B antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
LCE2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-99 amino acids from the C-terminal region of human LCE2B.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(LCE2B Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the LCE2B antibody detected the LCE2B protein (arrow).)
Western Blot (WB)
(LCE2B Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the LCE2B antibody detected the LCE2B protein (arrow).)
Flow Cytometry (FC/FACS)
(LCE2B Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(LCE2B Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-LCE2B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,219 Da
NCBI Official Full Name
late cornified envelope protein 2B
NCBI Official Synonym Full Names
late cornified envelope 2B
NCBI Official Symbol
LCE2B
NCBI Official Synonym Symbols
XP5; LEP10; SPRL1B
NCBI Protein Information
late cornified envelope protein 2B; late envelope protein 10; skin-specific protein Xp5; small proline rich-like (epidermal differentiation complex) 1B; small proline-rich-like epidermal differentiation complex protein 1B
UniProt Protein Name
Late cornified envelope protein 2B
Protein Family
UniProt Gene Name
LCE2B
UniProt Synonym Gene Names
LEP10; SPRL1B; XP5
UniProt Entry Name
LCE2B_HUMAN
NCBI Description
This gene is one of the at least 20 genes expressed during epidermal differentiation and located on chromosomal band 1q21. This gene is involved in epidermal differentiation, and it is expressed at high levels in normal and psoriatic skin, but not in cultured keratinocytes or in any other tested cell types or tissues. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Precursors of the cornified envelope of the stratum corneum.
Tissue specificity: Skin-specific. Expression was readily detected in adult trunk skin, adult arm skin, fetal skin, penal skin, vulva, esophagus and tongue. Not expressed in the cervix, rectum, lung, colon, or placenta. Ref.1 Ref.4
Induction: By calcium and UVB. Ref.4
Miscellaneous: Belongs to the LCE cluster present on 1q21.
Sequence similarities: Belongs to the LCE family.
Research Articles on LCE2B
Similar Products
Product Notes
The LCE2B lce2b (Catalog #AAA6315452) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LCE2B, CT (LCE2B, LEP10, SPRL1B, XP5, Late cornified envelope protein 2B, Late envelope protein 10, Skin-specific protein Xp5, Small proline-rich-like epidermal differentiation complex protein 1B) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LCE2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the LCE2B lce2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LCE2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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