Rabbit anti-Human KLK15 Polyclonal Antibody | anti-KLK15 antibody

KLK15, ID (KLK15, Kallikrein-15, ACO protease) (Biotin)

Cat. Num. AAA6314295

• Gene Names: KLK15; ACO; HSRNASPH
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KLK15; Polyclonal Antibody; ID (KLK15; Kallikrein-15; ACO protease) (Biotin); ACO protease; anti-KLK15 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-KLK15 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLK15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-106 amino acids from the Central region of human KLK15.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(KLK15 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the KLK15 antibody detected the KLK15 protein (arrow).)

Immunohistochemistry (IHC)

(KLK15 Antibody (Center) (AP14825c)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KLK15 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-KLK15 antibody

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. In prostate cancer, this gene has increased expression, which indicates its possible use as a test or prognostic marker for prostate cancer. The gene contains multiple polyadenylation sites and alternative splicing results in multiple transcript variants encoding distinct isoforms.

Product Categories/Family for anti-KLK15 antibody

Antibodies; Kallikrein

References

Rabien, A., et al. Int. J. Cancer 127(10):2386-2394(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Han, S., et al. Hum. Immunol. 71(7):727-730(2010). Gallagher, D.J., et al. Clin. Cancer Res. 16(10):2819-2832(2010). Klein, R.J., et al. Cancer Prev Res (Phila) 3(5):611-619(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 20302143
• NCBI GeneID: 55554
• NCBI Accession #: NP_059979.2; NP_612631.1
• NCBI GenBank Nucleotide #: NM_017509.3
• UniProt Accession #: Q9H2R5; Q15358; Q6ISI0; Q9H2R3; Q9H2R4; Q9H2R6; Q9HBG9; A0AUY8
• Molecular Weight: 28,016 Da
• NCBI Official Full Name: kallikrein-15 isoform 4 preproprotein
• NCBI Official Synonym Full Names: kallikrein-related peptidase 15
• NCBI Official Symbol: KLK15
• NCBI Official Synonym Symbols: ACO; HSRNASPH
• NCBI Protein Information: kallikrein-15; ACO protease; kallikrein-like serine protease; prostinogen
• UniProt Protein Name: Kallikrein-15
• Protein Family: Kallikrein
• UniProt Gene Name: KLK15
• UniProt Entry Name: KLK15_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. In prostate cancer, this gene has increased expression, which indicates its possible use as a diagnostic or prognostic marker for prostate cancer. The gene contains multiple polyadenylation sites and alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

KLK15: Protease whose physiological substrate is not yet known. Belongs to the peptidase S1 family. Kallikrein subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Protease; EC 3.4.21.-

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: extracellular region

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: proteolysis