Rabbit anti-Human ITGB1BP1 Polyclonal Antibody | anti-ITGB1BP1 antibody

ITGB1BP1, ID (ITGB1BP1, ICAP1, Integrin beta-1-binding protein 1, Integrin cytoplasmic domain-associated protein 1) (Biotin)

Cat. Num. AAA6311248

• Gene Names: ITGB1BP1; ICAP1; ICAP1A; ICAP1B; ICAP-1A; ICAP-1B; ICAP-1alpha
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ITGB1BP1; Polyclonal Antibody; ID (ITGB1BP1; ICAP1; Integrin beta-1-binding protein 1; Integrin cytoplasmic domain-associated protein 1) (Biotin); Integrin cytoplasmic domain-associated protein 1; anti-ITGB1BP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ITGB1BP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ITGB1BP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-136 amino acids from the Central region of human ITGB1BP1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ITGB1BP1 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the ITGB1BP1 antibody detected the ITGB1BP1 protein (arrow).)

Related Product Information for anti-ITGB1BP1 antibody

The cytoplasmic domains of integrins are essential for cell adhesion. The protein encoded by this gene binds to the beta1 integrin cytoplasmic domain. The interaction between this protein and beta1 integrin is highly specific. Two isoforms of this protein are derived from alternatively spliced transcripts. The shorter form of this protein does not interact with the beta1 integrin cytoplasmic domain. The longer form is a phosphoprotein and the extent of its phosphorylation is regulated by the cell-matrix interaction, suggesting an important role of this protein during integrin-dependent cell adhesion.

Product Categories/Family for anti-ITGB1BP1 antibody

Antibodies; Integrin

References

Brutsch, R., et al. Circ. Res. 107(5):592-601(2010). Mavaddat, N., et al. Cancer Epidemiol. Biomarkers Prev. 18(1):255-259(2009). Zhang, J., et al. Neurosurgery 63(3):571-578(2008). Furusu, A., et al. J. Cell. Physiol. 210(3):703-710(2007). Stroeken, P.J., et al. J. Cell. Physiol. 208(3):620-628(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 4758578
• NCBI GeneID: 9270
• NCBI Accession #: NP_004754.1
• NCBI GenBank Nucleotide #: NM_004763.3
• UniProt Accession #: O14713; O14714; Q53RS0; D6W4Y9
• Molecular Weight: 16,140 Da
• NCBI Official Full Name: integrin beta-1-binding protein 1 isoform 1
• NCBI Official Synonym Full Names: integrin beta 1 binding protein 1
• NCBI Official Symbol: ITGB1BP1
• NCBI Official Synonym Symbols: ICAP1; ICAP1A; ICAP1B; ICAP-1A; ICAP-1B; ICAP-1alpha
• NCBI Protein Information: integrin beta-1-binding protein 1; ICAP-1; bodenin; integrin cytoplasmic domain-associated protein 1; integrin cytoplasmic domain-associated protein 1-beta; integrin cytoplasmic domain-associated protein 1-alpha
• UniProt Protein Name: Integrin beta-1-binding protein 1
• Protein Family: Integrin beta-1-binding protein
• UniProt Gene Name: ITGB1BP1
• UniProt Synonym Gene Names: ICAP1; ICAP-1
• UniProt Entry Name: ITBP1_HUMAN

NCBI Description

The cytoplasmic domains of integrins are essential for cell adhesion. The protein encoded by this gene binds to the beta1 integrin cytoplasmic domain. The interaction between this protein and beta1 integrin is highly specific. Two isoforms of this protein are derived from alternatively spliced transcripts. The shorter form of this protein does not interact with the beta1 integrin cytoplasmic domain. The longer form is a phosphoprotein and the extent of its phosphorylation is regulated by the cell-matrix interaction, suggesting an important role of this protein during integrin-dependent cell adhesion. [provided by RefSeq, Jul 2008]

Uniprot Description

ICAP1: May play a role in the recruitment of beta-1 integrins to the focal contacts during integrin-dependent cell adhesion. Isoform 2 does not bind the integrin cytoplasmic domain-associated protein-1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p25.2

Cellular Component: ruffle; focal adhesion; cytoskeleton; membrane; perinuclear region of cytoplasm; lamellipodium; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: integrin binding; protein binding; GDP-dissociation inhibitor activity; protein complex binding; protein kinase binding; protein transporter activity

Biological Process: integrin activation; cell-matrix adhesion; receptor clustering; negative regulation of cell proliferation; regulation of cell adhesion mediated by integrin; protein transport; positive regulation of focal adhesion formation; biomineral formation; positive regulation of cell proliferation; positive regulation of stress fiber formation; cell differentiation; regulation of blood vessel size; negative regulation of protein binding; positive regulation of Notch signaling pathway; integrin-mediated signaling pathway; Notch signaling pathway; cell migration; substrate-bound cell migration, cell release from substrate; transcription, DNA-dependent; activation of protein kinase B; negative regulation of focal adhesion formation; lumen formation; positive regulation of protein kinase B signaling cascade; blood vessel endothelial cell proliferation during sprouting angiogenesis; positive regulation of cell division; myoblast migration; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter