Rabbit anti-Human, Mouse ITGA2 Polyclonal Antibody | anti-ITGA2 antibody

ITGA2, CT (ITGA2, CD49B, Integrin alpha-2, CD49 antigen-like family member B, Collagen receptor, Platelet membrane glycoprotein Ia, VLA-2 subunit alpha, CD49b) (FITC)

Cat. Num. AAA6311150

• Gene Names: ITGA2; BR; GPIa; CD49B; HPA-5; VLA-2; VLAA2
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ITGA2; Polyclonal Antibody; CT (ITGA2; CD49B; Integrin alpha-2; CD49 antigen-like family member B; Collagen receptor; Platelet membrane glycoprotein Ia; VLA-2 subunit alpha; CD49b) (FITC); CD49b; anti-ITGA2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ITGA2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ITGA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1151-1180 amino acids from the C-terminal region of human ITGA2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ITGA2 Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the ITGA2 antibody detected the ITGA2 protein (arrow).)

Western Blot (WB)

(ITGA2 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the ITGA2 antibody detected the ITGA2 protein (arrow).)

Related Product Information for anti-ITGA2 antibody

This gene product belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane glycoproteins composed of a distinct alpha chain and a common beta chain. They are found on a wide variety of cell types including, T cells, fibroblasts and platelets. Integrins are involved in cell adhesion and also participate in cell-surface mediated signalling. [provided by RefSeq].

Product Categories/Family for anti-ITGA2 antibody

Antibodies; CD Markers

References

Nie, Y.M., et al. Transfus Med 20(6):376-382(2010). Motovska, Z., et al. Atherosclerosis 212(2):548-552(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Hozumi, K., et al. FEBS Lett. 584(15):3381-3385(2010). Wang, Y., et al. Diabet. Med. 27(4):376-383(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 116295258
• NCBI GeneID: 3673
• NCBI Accession #: NP_002194.2
• NCBI GenBank Nucleotide #: NM_002203.3
• UniProt Accession #: P17301; Q14595
• Molecular Weight: 129,295 Da
• NCBI Official Full Name: integrin alpha-2
• NCBI Official Synonym Full Names: integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
• NCBI Official Symbol: ITGA2
• NCBI Official Synonym Symbols: BR; GPIa; CD49B; HPA-5; VLA-2; VLAA2
• NCBI Protein Information: integrin alpha-2; collagen receptor; platelet antigen Br; platelet glycoprotein GPIa; VLA2 receptor, alpha-2 subunit; CD49 antigen-like family member B; platelet membrane glycoprotein Ia; human platelet alloantigen system 5; very late activation protein 2
• UniProt Protein Name: Integrin alpha-2
• Protein Family: Integrin
• UniProt Gene Name: ITGA2
• UniProt Synonym Gene Names: CD49B; GPIa
• UniProt Entry Name: ITA2_HUMAN

NCBI Description

This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

ITGA2: Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Belongs to the integrin alpha chain family.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: cell surface; focal adhesion; perinuclear region of cytoplasm; plasma membrane; nerve terminal; integrin complex; nucleus; external side of plasma membrane

Molecular Function: integrin binding; collagen binding; viral receptor activity; protein binding; protein heterodimerization activity; metal ion binding; laminin binding

Biological Process: axon guidance; entry of virus into host cell; extracellular matrix organization and biogenesis; response to muscle activity; positive regulation of positive chemotaxis; positive regulation of cell adhesion; positive regulation of translation; positive regulation of leukocyte migration; cell-matrix adhesion; positive regulation of smooth muscle cell proliferation; positive regulation of collagen binding; positive regulation of collagen biosynthetic process; positive regulation of cell projection organization and biogenesis; mesodermal cell differentiation; positive regulation of smooth muscle cell migration; response to L-ascorbic acid; response to organic cyclic substance; establishment of protein localization; mammary gland development; positive regulation of smooth muscle contraction; cell adhesion; positive regulation of DNA binding; substrate-bound cell migration; integrin-mediated signaling pathway; response to drug; skin morphogenesis; hypotonic response; cell-substrate adhesion; cellular response to hormone stimulus; cell proliferation; detection of mechanical stimulus involved in sensory perception of pain; organ morphogenesis; response to hypoxia; cell adhesion mediated by integrin; positive regulation of phagocytosis, engulfment; blood coagulation; positive regulation of transmission of nerve impulse; positive regulation of inflammatory response; response to amine stimulus

Disease: Bleeding Disorder, Platelet-type, 9