Rabbit anti-Human ABI1 Polyclonal Antibody | anti-ABI1 antibody

ABI1, NT (Abl Interactor 1, Abelson Interactor 1, Abi-1, Spectrin SH3 Domain-binding Protein 1, Eps8 SH3 Domain-binding Protein, Eps8-binding Protein, e3B1, Nap1-binding Protein, Nap1BP, Abl-binding Protein 4, AblBP4, SSH3BP1)

Cat. Num. AAA631106

• Gene Names: ABI1; ABA INSENSITIVE 1; ABI1; AtABI1; F20B18.190; F20B18_190; PROTEIN PHOSPHATASE 2C ABI1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ABI1; Polyclonal Antibody; NT (Abl Interactor 1; Abelson Interactor 1; Abi-1; Spectrin SH3 Domain-binding Protein 1; Eps8 SH3 Domain-binding Protein; Eps8-binding Protein; e3B1; Nap1-binding Protein; Nap1BP; Abl-binding Protein 4; AblBP4; SSH3BP1); Anti -ABI1; anti-ABI1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ABI1.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-ABI1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500
• Immunogen: Synthetic peptide selected from the N-terminal region of human ABI1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ABI1 Antibody (N-term) in MCF-7, CEM, Jurkat cell line lysates (35ug/lane). ABI1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with ABI1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of SK-Br-3 cells using ABI1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Immunofluorescence analysis of ABI1 Antibody (N-term) with paraffin-embedded human brain tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.)

Related Product Information for anti-ABI1 antibody

ABI1 has been found to form a complex with EPS8 and SOS1, and is thought to be involved in the transduction of signals from Ras to Rac. In addition, this protein may play a role in the regulation of EGF-induced Erk pathway activation as well as cytoskeletal reorganization and EGFR signaling.

Product Categories/Family for anti-ABI1 antibody

Antibodies; Abs to Spectrin

NCBI and Uniprot Product Information

• NCBI GI #: 499301
• NCBI GeneID: 828714
• UniProt Accession #: P49597; Q0WW30; Q43717; Q94C87
• Molecular Weight: 47,506 Da
• NCBI Official Full Name: ABI1
• NCBI Official Symbol: ABI1
• NCBI Official Synonym Symbols: ABA INSENSITIVE 1; ABI1; AtABI1; F20B18.190; F20B18_190; PROTEIN PHOSPHATASE 2C ABI1
• NCBI Protein Information: protein phosphatase 2C 56
• UniProt Protein Name: Protein phosphatase 2C 56
• Protein Family: Abl interactor
• UniProt Gene Name: ABI1
• UniProt Entry Name: P2C56_ARATH

NCBI Description

Involved in abscisic acid (ABA) signal transduction. Negative regulator of ABA promotion of stomatal closure.

Uniprot Description

Function: Key component and repressor of the abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomatal closure, osmotic water permeability of the plasma membrane (Pos), drought-induced resistance and rhizogenesis, response to glucose, high light stress, seed germination and inhibition of vegetative growth. During the stomatal closure regulation, modulates the inward calcium-channel permeability as well as the actin reorganization in guard cells in response to ABA. Involved in the resistance to the bacterial pathogen Pseudomonas syringae pv. tomato. Controls negatively fibrillin expression that is involved in mediating ABA-induced photoprotection. May be involved in ABA content regulation. Plays a role in the Pro accumulation in response to reduced water availability (low water potential). Required for the ABA negative regulation of the ethylene-induced hyponastic growth. Involved in acquired thermotolerance of root growth and seedling survival. Activates/represses SRK2E/OST1 in response to ABA-dependent stimuli, especially in stomata closure regulation involving SLAC1. Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24 Ref.26 Ref.27 Ref.28 Ref.29 Ref.30 Ref.31 Ref.32 Ref.33 Ref.34 Ref.35 Ref.37 Ref.38 Ref.39 Ref.40 Ref.41 Ref.42 Ref.43 Ref.44 Ref.45 Ref.47 Ref.48 Ref.50 Ref.51 Ref.52 Ref.53 Ref.55 Ref.57 Ref.58 Ref.60 Ref.61 Ref.63 Ref.66

Catalytic activity: A phosphoprotein + H2O = a protein + phosphate. Ref.16 Ref.25 Ref.27

Cofactor: Binds 2 magnesium or manganese ions per subunit. Ref.16 Ref.25

Enzyme regulation: Phosphatase activity repressed by oxidized GPX3 and phosphatidic acid (PA). PA is produced by PLD alpha 1 in response to ABA. Repressed by PYR/PYL/RCAR ABA receptors in an ABA-dependent manner. Ref.48 Ref.58 Ref.68 Ref.70

Subunit structure: Interacts with ATHB-6, CIPK15/PKS3, GPX3, SRK2E/OST1, SRK2D, SRK2I, SCAR1, SCAR2, SCAR3 and SCARL. Binds to the PA released by the phospholipase D alpha 1 (PLDALPHA1) in response to ABA during the stomatal closure regulation. Interacts with ABA-bounded PYR1, PYL1, PYL2, PYL3, PYL4, PYL5, PYL6, PYL7, PYL8, PYL9, PYL10, and with free PYL2, PYL3 and PYL4. Binds to RPL12B, CPK21 and CPK23. Ref.38 Ref.39 Ref.48 Ref.52 Ref.54 Ref.58 Ref.59 Ref.65 Ref.66 Ref.67 Ref.68 Ref.69 Ref.70 Ref.71 Ref.72 Ref.73

Subcellular location: Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein. Note: Associated to the plasma membrane when in complex with PA, subsequently to ABA signaling. Ref.48 Ref.63

Tissue specificity: Expressed in seeds and seedlings. In roots, confined to lateral root caps and columella cells. Ref.56 Ref.63

Induction: Repressed by MYB44. Induced by low temperature, drought, high salt, ABA and ethylene. Ref.36 Ref.46 Ref.48 Ref.56 Ref.58 Ref.61 Ref.64 Ref.68 Ref.70

Domain: The 'lock' site stabilizes the complex made of PP2C, ABA and PYR/PYL/RCAR receptor by keeping receptor 'gate' and 'latch' loops in closed positions.

Miscellaneous: Enhanced ABA signaling repressor activity by the proteasomal inhibitor MG132 accompanied by a cytoplasmic localization.Plants insensitive to ABA (abi1-1) are more resitant to P.syringae.

Sequence similarities: Belongs to the PP2C family.Contains 1 PP2C-like domain.

Biophysicochemical propertiespH dependence:Optimum pH is 8. Ref.25