Rabbit anti-Human IRAK4 Polyclonal Antibody | anti-IRAK4 antibody

IRAK4, NT (IRAK4, Interleukin-1 receptor-associated kinase 4, Renal carcinoma antigen NY-REN-64) (APC)

Cat. Num. AAA6310862

• Gene Names: IRAK4; IPD1; REN64; NY-REN-64
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: IRAK4; Polyclonal Antibody; NT (IRAK4; Interleukin-1 receptor-associated kinase 4; Renal carcinoma antigen NY-REN-64) (APC); Renal carcinoma antigen NY-REN-64; anti-IRAK4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IRAK4.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-IRAK4 antibody: FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 25-52 in the N-terminal region of human IRAK4, UniProt Accession # NP_001107654.1; NP_057207.2. Species sequence homology: bovine, mouse
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) using 037098. This demonstrates that 037098 detected the IRAK4 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in K562 whole cell lysate (20ugllane) using 037098 (1:1000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 52kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-IRAK4 antibody

This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-IRAK4 antibody

Antibodies; Protein Kinases

References

1. Silva, L.K., et al. Eur. J. Hum. Genet. 18(11):1221-1227(2010). 2. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 3. McDonald, D.R., et al. J. Allergy Clin. Immunol. 126(2):332-337(2010). 4. Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010). 5. Wang, Z., et al. Structure 14(12):1835-1844(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 166795295
• NCBI GeneID: 51135
• NCBI Accession #: NP_001107654.1; NP_057207.2
• NCBI GenBank Nucleotide #: NM_001114182.2
• UniProt Accession #: Q9NWZ3; Q69FE3; Q7Z6A7; Q7Z6A8
• Molecular Weight: 51,530 Da
• NCBI Official Full Name: interleukin-1 receptor-associated kinase 4 isoform a
• NCBI Official Synonym Full Names: interleukin-1 receptor-associated kinase 4
• NCBI Official Symbol: IRAK4
• NCBI Official Synonym Symbols: IPD1; REN64; NY-REN-64
• NCBI Protein Information: interleukin-1 receptor-associated kinase 4; IRAK-4; renal carcinoma antigen NY-REN-64
• UniProt Protein Name: Interleukin-1 receptor-associated kinase 4
• Protein Family: Interleukin-1 receptor-associated kinase
• UniProt Gene Name: IRAK4
• UniProt Synonym Gene Names: IRAK-4
• UniProt Entry Name: IRAK4_HUMAN

NCBI Description

This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [supplied by RefSeq]

Uniprot Description

IRAK4: a TKL kinase of the IRAK family. The interleukin-1 receptor-associated kinases are important mediators in the signal transduction of Toll-like receptor and IL1R family members, collectively referred to as TIRs.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, TKL; TKL group; IRAK family

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: extracellular space; cytoplasm; plasma membrane; endosome membrane; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; interleukin-1 receptor binding; magnesium ion binding; ATP binding; protein kinase activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of smooth muscle cell proliferation; cytokine and chemokine mediated signaling pathway; cytokine production; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor signaling pathway; innate immune response; JNK cascade; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway

Disease: Invasive Pneumococcal Disease, Recurrent Isolated, 1; Irak4 Deficiency