Rabbit anti-Mouse Irak3 Polyclonal Antibody | anti-Irak3 antibody

Irak3, CT (Irak3, Interleukin-1 receptor-associated kinase 3, IL-1 receptor-associated kinase M) (Azide free) (HRP)

Cat. Num. AAA6310843

• Gene Names: Irak3; IRAK-M; AI563835; 4833428C18Rik
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Irak3; Polyclonal Antibody; CT (Irak3; Interleukin-1 receptor-associated kinase 3; IL-1 receptor-associated kinase M) (Azide free) (HRP); IL-1 receptor-associated kinase M; anti-Irak3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Irak3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Irak3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-544 amino acids from the C-terminal region of Mouse Irak3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Irak3 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Irak3 antibody detected the Irak3 protein (arrow).)

Related Product Information for anti-Irak3 antibody

Irak3 inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex.

Product Categories/Family for anti-Irak3 antibody

Antibodies; Protein Kinases

References

Turnis, M.E., et al. J. Immunol. 185(7):4223-4232(2010). Hubbard, L.L., et al. J. Immunol. 184(11):6299-6308(2010). Seki, M., et al. J. Immunol. 184(3):1410-1418(2010). Lyn-Kew, K., et al. PLoS ONE 5 (6), E11145 (2010): Su, J., et al. J Innate Immun 1(2):164-174(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 31543005
• NCBI GeneID: 73914
• NCBI Accession #: NP_082955.2
• NCBI GenBank Nucleotide #: NM_028679.3
• UniProt Accession #: Q8K4B2; Q8C7U8; Q8CE40; Q8K1S8
• NCBI Official Full Name: interleukin-1 receptor-associated kinase 3
• NCBI Official Synonym Full Names: interleukin-1 receptor-associated kinase 3
• NCBI Official Symbol: Irak3
• NCBI Official Synonym Symbols: IRAK-M; AI563835; 4833428C18Rik
• NCBI Protein Information: interleukin-1 receptor-associated kinase 3
• UniProt Protein Name: Interleukin-1 receptor-associated kinase 3
• Protein Family: Interleukin-1 receptor-associated kinase
• UniProt Gene Name: Irak3
• UniProt Synonym Gene Names: IRAK-3; IRAK-M
• UniProt Entry Name: IRAK3_MOUSE

Uniprot Description

IRAK3: a TKL kinase of the IRAK family. The interleukin-1 receptor-associated kinases are important mediators in the signal transduction of Toll-like receptor and IL1R family members, collectively referred to as TIRs.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; EC 2.7.11.1; TKL group; IRAK family

Cellular Component: interleukin-1 receptor complex; cytoplasm; nucleus

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; protein heterodimerization activity; transferase activity, transferring phosphorus-containing groups; magnesium ion binding; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: negative regulation of MAP kinase activity; regulation of protein complex disassembly; negative regulation of interleukin-12 production; apoptosis; cytokine and chemokine mediated signaling pathway; response to virus; protein amino acid autophosphorylation; response to lipopolysaccharide; negative regulation of tumor necrosis factor production; signal transduction; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; regulation of apoptosis; response to exogenous dsRNA; inhibition of NF-kappaB transcription factor; negative regulation of innate immune response; response to peptidoglycan; negative regulation of interleukin-6 production; negative regulation of toll-like receptor signaling pathway; negative regulation of cytokine and chemokine mediated signaling pathway; negative regulation of protein catabolic process; phosphorylation; negative regulation of protein complex disassembly