Western Blot (WB)
(HSD17B1 Antibody (C-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the HSD17B1 antibody detected the HSD17B1 protein (arrow).)
Rabbit anti-Human HSD17B1 Polyclonal Antibody | anti-HSD17B1 antibody
HSD17B1, CT (HSD17B1, E17KSR, EDH17B1, EDH17B2, EDHB17, Estradiol 17-beta-dehydrogenase 1, 17-beta-hydroxysteroid dehydrogenase type 1, 20 alpha-hydroxysteroid dehydrogenase, E2DH, Placental 17-beta-hydroxysteroid dehydrogenase) (APC)
Gene Names
HSD17B1; HSD17; EDHB17; EDH17B2; SDR28C1
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
HSD17B1; Polyclonal Antibody; CT (HSD17B1; E17KSR; EDH17B1; EDH17B2; EDHB17; Estradiol 17-beta-dehydrogenase 1; 17-beta-hydroxysteroid dehydrogenase type 1; 20 alpha-hydroxysteroid dehydrogenase; E2DH; Placental 17-beta-hydroxysteroid dehydrogenase) (APC); Placental 17-beta-hydroxysteroid dehydrogenase; anti-HSD17B1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-HSD17B1 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
HSD17B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-256 amino acids from the C-terminal region of human HSD17B1.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(HSD17B1 Antibody (C-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the HSD17B1 antibody detected the HSD17B1 protein (arrow).)
Western Blot (WB)
(HSD17B1 Antibody (C-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the HSD17B1 antibody detected the HSD17B1 protein (arrow).)
Related Product Information for anti-HSD17B1 antibody
Favors the reduction of estrogens and androgens. Also has 20-alpha-HSD activity. Uses preferentially NADH.
Product Categories/Family for anti-HSD17B1 antibody
References
Canzian, F., et al. Hum. Mol. Genet. 19(19):3873-3884(2010). Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010). Lamp, M., et al. Gynecol. Endocrinol. (2010). In press: Haller, F., et al. J. Mol. Biol. 399(2):255-267(2010). Iwasaki, M., et al. Nutr Cancer 62(4):466-475(2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,950 Da
NCBI Official Full Name
estradiol 17-beta-dehydrogenase 1
NCBI Official Synonym Full Names
hydroxysteroid (17-beta) dehydrogenase 1
NCBI Official Symbol
HSD17B1
NCBI Official Synonym Symbols
HSD17; EDHB17; EDH17B2; SDR28C1
NCBI Protein Information
estradiol 17-beta-dehydrogenase 1; 17-beta-HSD 1; 17-beta-hydroxysteroid dehydrogenase type 1; 20 alpha-hydroxysteroid dehydrogenase; 20-alpha-HSD; E2DH; estradiol 17-beta-dehydrogenase-1; hydroxysteroid (17-beta) dehydrogenase 1 isoform; placental 17-bet
UniProt Protein Name
Estradiol 17-beta-dehydrogenase 1
Protein Family
UniProt Gene Name
HSD17B1
UniProt Synonym Gene Names
E17KSR; EDH17B1; EDH17B2; EDHB17; 17-beta-HSD 1; 20-alpha-HSD
UniProt Entry Name
DHB1_HUMAN
Uniprot Description
HSD17B1: Favors the reduction of estrogens and androgens. Also has 20-alpha-HSD activity. Uses preferentially NADH. Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Protein type: Lipid Metabolism - androgen and estrogen; EC 1.1.1.62; Oxidoreductase
Chromosomal Location of Human Ortholog: 17q11-q21
Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; cytosol
Molecular Function: estradiol 17-beta-dehydrogenase activity; catalytic activity; 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity
Biological Process: steroid metabolic process; estrogen metabolic process; steroid biosynthetic process; estrogen biosynthetic process
Research Articles on HSD17B1
Similar Products
Product Notes
The HSD17B1 hsd17b1 (Catalog #AAA6308453) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSD17B1, CT (HSD17B1, E17KSR, EDH17B1, EDH17B2, EDHB17, Estradiol 17-beta-dehydrogenase 1, 17-beta-hydroxysteroid dehydrogenase type 1, 20 alpha-hydroxysteroid dehydrogenase, E2DH, Placental 17-beta-hydroxysteroid dehydrogenase) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSD17B1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HSD17B1 hsd17b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSD17B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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