Rabbit anti-Human HNRNPA1 Polyclonal Antibody | anti-HNRNPA1 antibody

HNRNPA1, CT (HNRNPA1, HNRPA1, Heterogeneous nuclear ribonucleoprotein A1, Helix-destabilizing protein, Single-strand RNA-binding protein, hnRNP core protein A1) (PE)

Cat. Num. AAA6307450

• Gene Names: HNRNPA1; HNRPA1; HNRPA1L3; hnRNP A1; hnRNP-A1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HNRNPA1; Polyclonal Antibody; CT (HNRNPA1; HNRPA1; Heterogeneous nuclear ribonucleoprotein A1; Helix-destabilizing protein; Single-strand RNA-binding protein; hnRNP core protein A1) (PE); hnRNP core protein A1; anti-HNRNPA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-HNRNPA1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: HNRNPA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-294 amino acids from the C-terminal region of human HNRNPA1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(HNRNPA1 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HNRNPA1 antibody detected the HNRNPA1 protein (arrow).)

Western Blot (WB)

(Western blot analysis of HNRNPA1 (arrow) using rabbit polyclonal HNRNPA1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HNRNPA1 gene.)

Related Product Information for anti-HNRNPA1 antibody

This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for this gene but only two transcripts are fully described. These variants have multiple alternative transcription initiation sites and multiple polyA sites. [provided by RefSeq].

Product Categories/Family for anti-HNRNPA1 antibody

Antibodies; Nuclear Proteins

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Michlewski, G., et al. Nat. Struct. Mol. Biol. 17(8):1011-1018(2010). Clower, C.V., et al. Proc. Natl. Acad. Sci. U.S.A. 107(5):1894-1899(2010). David, C.J., et al. Nature 463(7279):364-368(2010). Fisette, J.F., et al. RNA 16(1):228-238(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4504445
• NCBI GeneID: 3178
• NCBI Accession #: NP_002127.1; NP_112420.1
• NCBI GenBank Nucleotide #: NM_002136.2
• UniProt Accession #: P09651; Q3MIB7; Q6PJZ7; A8K4Z8
• Molecular Weight: 38,747 Da
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein A1 isoform a
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein A1
• NCBI Official Symbol: HNRNPA1
• NCBI Official Synonym Symbols: HNRPA1; HNRPA1L3; hnRNP A1; hnRNP-A1
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein A1; hnRNP A1-like 3; hnRNP core protein A1; helix-destabilizing protein; hnRNP core protein A1-like 3; single-strand RNA-binding protein; single-strand DNA-binding protein UP1; nuclear ribonucleoprotein particle A1
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein A1
• Protein Family: Heterogeneous nuclear ribonucleoproteins
• UniProt Gene Name: HNRNPA1
• UniProt Synonym Gene Names: HNRPA1; hnRNP A1
• UniProt Entry Name: ROA1_HUMAN

NCBI Description

This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for this gene but only two transcripts are fully described. These variants have multiple alternative transcription initiation sites and multiple polyA sites. [provided by RefSeq, Jul 2008]

Uniprot Description

hnRNP A1: Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication. Identified in the spliceosome C complex. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with SEPT6. Interacts with HCV NS5B and with the 5'-UTR and 3'-UTR of HCV RNA. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Spliceosome; RNA splicing; Nuclear export

Chromosomal Location of Human Ortholog: 12q13.1

Cellular Component: nucleoplasm; spliceosome; intermediate filament cytoskeleton; membrane; cytoplasm; ribonucleoprotein complex

Molecular Function: protein binding; single-stranded RNA binding; RNA binding; nucleotide binding; single-stranded DNA binding

Biological Process: nuclear mRNA splicing, via spliceosome; mRNA transport; viral reproduction; RNA export from nucleus; RNA splicing; nuclear import; nuclear export; gene expression; mRNA processing

Disease: Inclusion Body Myopathy With Early-onset Paget Disease With Or Without Frontotemporal Dementia 3; Amyotrophic Lateral Sclerosis 20