Rabbit anti-Human VGLUT3 Polyclonal Antibody | anti-SLC17A8 antibody

VGLUT3 (Vesicular Glutamate Transporter 3, Solute Carrier Family 17 Member 8, SLC17A8)

Cat. Num. AAA630740

• Gene Names: SLC17A8; DFNA25; VGLUT3
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: VGLUT3; Polyclonal Antibody; VGLUT3 (Vesicular Glutamate Transporter 3; Solute Carrier Family 17 Member 8; SLC17A8); Anti -VGLUT3 (Vesicular Glutamate Transporter 3; anti-SLC17A8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human VGLUT3.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul of sterile PBS.

• Applicable Applications for anti-SLC17A8 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot and ELISA.; Dilution: Western Blot: 1-10ug/ml (Chemiluminescence); ELISA: 1:100,000; 50-100ng of G4007-02A control peptide/well
• Immunogen: Synthetic peptide corresponding to 18aa near the N-terminal, cytoplasmic domain of human VGLUT3 conjugated to KLH (Q8NDX2). Species Sequence Homology (100%): rat and mouse
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-SLC17A8 antibody

Vesicular glutamate transporter (VGLUT) is responsible for the active transport of L-glutamate in synaptic vesicles and thus plays an essential role in the glutamatergic chemical transmission in the central nervous system. VGLUT comprises three isoforms, VGLUT1, 2, and 3, and is a potential marker for the glutamatergic phenotype. Recent studies indicated that VGLUT is also expressed in non-neuronal cells, and localized with various organelles such as synaptic-like microvesicles in the pineal gland, and hormone- containing secretory granules in endocrine cells. L-Glutamate is stored in these organelles, secreted upon various forms of stimulation, and then acts as a paracrine-like modulator. Thus, VGLUTs highlight a novel framework of glutamatergic signaling and reveal its diverse modes of action. VGLUT3 expression is restricted to the brain, where it is predominantly located in synaptic vesicles. VGLUT3 shows strong sequence homology (~73%) to Differentiation-associated Na- dependent inorganic phosphate cotransporter. Mouse and rat respectively show 90 and 92% identity with the human VGLUT3 protein sequence.

Product Categories/Family for anti-SLC17A8 antibody

Antibodies; Abs to Transport Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 223718438
• NCBI GeneID: 246213
• NCBI Accession #: NP_001138760.1
• NCBI GenBank Nucleotide #: NM_001145288.1
• UniProt Accession #: Q8NDX2; Q17RQ8; B3KXZ6; B7ZKV4
• Molecular Weight: 64,991 Da
• NCBI Official Full Name: vesicular glutamate transporter 3 isoform 2
• NCBI Official Synonym Full Names: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 8
• NCBI Official Symbol: SLC17A8
• NCBI Official Synonym Symbols: DFNA25; VGLUT3
• NCBI Protein Information: vesicular glutamate transporter 3; solute carrier family 17 member 8
• UniProt Protein Name: Vesicular glutamate transporter 3
• Protein Family: Vesicular glutamate transporter
• UniProt Gene Name: SLC17A8
• UniProt Synonym Gene Names: VGLUT3
• UniProt Entry Name: VGLU3_HUMAN

NCBI Description

This gene encodes a vesicular glutamate transporter. The encoded protein transports the neurotransmitter glutamate into synaptic vesicles before it is released into the synaptic cleft. Mutations in this gene are the cause of autosomal-dominant nonsyndromic type 25 deafness. Alternate splicing results in multiple transcript variants.

Uniprot Description

SLC17A8: Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. Defects in SLC17A8 are the cause of deafness autosomal dominant type 25 (DFNA25). DFNA25 is a form of sensorineural hearing loss. The expression of DFNA25 deafness is variable in terms of onset and rate of progression, with an age- dependent penetrance resembling an early-onset presbycusis, or senile deafness, a progressive bilateral loss of hearing that occurs in the aged. Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, SLC family; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q23.1

Cellular Component: neuron projection; synaptic vesicle membrane; integral to membrane; cell junction

Molecular Function: symporter activity; L-glutamate transmembrane transporter activity

Biological Process: sensory perception of sound; neurotransmitter transport; sodium ion transport; ion transport; transmembrane transport

Disease: Deafness, Autosomal Dominant 25