Rabbit anti-Human HIST1H2AG Polyclonal Antibody | anti-HIST1H2AG antibody

HIST1H2AG, CT (HIST1H2AG, H2AFP, Histone H2A type 1, Histone H2A/p) (PE)

Cat. Num. AAA6306757

• Gene Names: HIST1H2AG; H2AG; H2A/p; H2AFP; H2A.1b; pH2A/f
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HIST1H2AG; Polyclonal Antibody; CT (HIST1H2AG; H2AFP; Histone H2A type 1; Histone H2A/p) (PE); Histone H2A/p; anti-HIST1H2AG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-HIST1H2AG antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa102-130 in the C-terminal region of human HIST1H2AG, UniProt Accession #NP_066408.1; P0C0S8. Species sequence homology: bovine, mouse and rat
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(HIST1H2AG Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the HIST1H2AG antibody detected the HIST1H2AG protein (arrow).)

Related Product Information for anti-HIST1H2AG antibody

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq].

Product Categories/Family for anti-HIST1H2AG antibody

Antibodies; Histones

References

Shi, J., et al. Nature 460(7256):753-757(2009). Nicassio, F., et al. Curr. Biol. 17(22):1972-1977(2007). Lusic, M., et al. EMBO J. 22(24):6550-6561(2003). Marzluff, W.F., et al. Genomics 80(5):487-498(2002). Deng, L., et al. Virology 289(2):312-326(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 10800132
• NCBI GeneID: 8969
• NCBI Accession #: NP_066408.1
• NCBI GenBank Nucleotide #: NM_021064.4
• UniProt Accession #: P0C0S8; P02261; Q2M1R2; Q76PA6
• Molecular Weight: 14,091 Da
• NCBI Official Full Name: histone H2A type 1
• NCBI Official Synonym Full Names: histone cluster 1, H2ag
• NCBI Official Symbol: HIST1H2AG
• NCBI Official Synonym Symbols: H2AG; H2A/p; H2AFP; H2A.1b; pH2A/f
• NCBI Protein Information: histone H2A type 1; H2A.1; histone H2A/p; histone 1, H2ag; H2A histone family, member P
• UniProt Protein Name: Histone H2A type 1
• UniProt Gene Name: HIST1H2AG
• UniProt Synonym Gene Names: H2AFP; H2A.1

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Jul 2008]

Uniprot Description

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.