Rabbit anti-Human HIST1H2AB Polyclonal Antibody | anti-HIST1H2AB antibody

HIST1H2AB, NT (HIST1H2AB, H2AFM, Histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a, Histone H2A/m) (AP)

Cat. Num. AAA6306725

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HIST1H2AB; Polyclonal Antibody; NT (HIST1H2AB; H2AFM; Histone H2A type 1-B/E; Histone H2A.2; Histone H2A/a; Histone H2A/m) (AP); Histone H2A/m; anti-HIST1H2AB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 130

• Applicable Applications for anti-HIST1H2AB antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: HIST1H2AB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-40 amino acids from the N-terminal region of human HIST1H2AB.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(HIST1H2AB Antibody (N-term) western blot analysis in K562,CEM,MDA-MB453,NCI-H460,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HIST1H2AB antibody detected the HIST1H2AB protein (arrow).)

Immunohistochemistry (IHC)

(HIST1H2AB Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HIST1H2AB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(HIST1H2AB Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-HIST1H2AB antibody

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Product Categories/Family for anti-HIST1H2AB antibody

Antibodies; Histones

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Bergink, S., et al. Genes Dev. 20(10):1343-1352(2006). Cao, R., et al. Mol. Cell 20(6):845-854(2005). Hagiwara, T., et al. Biochemistry 44(15):5827-5834(2005). Andersen, J.S., et al. Nature 433(7021):77-83(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 10645195
• NCBI Accession #: NP_066390.1
• NCBI GenBank Nucleotide #: NP_066390.1
• UniProt Accession #: P04908
• NCBI Official Full Name: histone H2A type 1-B/E
• UniProt Protein Name: Histone H2A type 1-B/E
• Protein Family: Histone
• UniProt Gene Name: HIST1H2AB
• UniProt Synonym Gene Names: H2AFM

Uniprot Description

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.