Rabbit anti-Human HIP1R Polyclonal Antibody | anti-HIP1R antibody

HIP1R, NT (HIP1R, HIP12, KIAA0655, Huntingtin-interacting protein 1-related protein, Huntingtin-interacting protein 12) (AP)

Cat. Num. AAA6306549

• Gene Names: HIP1R; HIP3; HIP12; ILWEQ
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HIP1R; Polyclonal Antibody; NT (HIP1R; HIP12; KIAA0655; Huntingtin-interacting protein 1-related protein; Huntingtin-interacting protein 12) (AP); Huntingtin-interacting protein 12; anti-HIP1R antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-HIP1R antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: HIP1R antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-133 amino acids from the N-terminal region of human HIP1R.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(HIP1R Antibody (N-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the HIP1R antibody detected the HIP1R protein (arrow).)

Immunohistochemistry (IHC)

(HIP1R Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HIP1R Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(HIP1R Antibody (N-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-HIP1R antibody

This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).

Product Categories/Family for anti-HIP1R antibody

Antibodies; KIAA; orf

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Kim, J.H., et al. Cell. Physiol. Biochem. 23 (1-3), 43-52 (2009): Wilbur, J.D., et al. J. Biol. Chem. 283(47):32870-32879(2008). Poupon, V., et al. Proc. Natl. Acad. Sci. U.S.A. 105(1):168-173(2008). Parker, J.A., et al. J. Neurosci. 27(41):11056-11064(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 48762942
• NCBI GeneID: 9026
• NCBI Accession #: NP_003950.1
• NCBI GenBank Nucleotide #: NM_003959.2
• UniProt Accession #: O75146; Q6NXG8; Q9UED9; A6NHQ6
• Molecular Weight: 70,288 Da
• NCBI Official Full Name: huntingtin-interacting protein 1-related protein isoform 1
• NCBI Official Synonym Full Names: huntingtin interacting protein 1 related
• NCBI Official Symbol: HIP1R
• NCBI Official Synonym Symbols: HIP3; HIP12; ILWEQ
• NCBI Protein Information: huntingtin-interacting protein 1-related protein
• UniProt Protein Name: Huntingtin-interacting protein 1-related protein
• Protein Family: Huntingtin-interacting protein 1-related protein
• UniProt Gene Name: HIP1R
• UniProt Synonym Gene Names: HIP12; KIAA0655; HIP1-related protein; HIP-12
• UniProt Entry Name: HIP1R_HUMAN

Uniprot Description

HIP1R: Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3- phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis. Belongs to the SLA2 family.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: cytoskeleton; intracellular membrane-bound organelle; clathrin-coated vesicle; perinuclear region of cytoplasm; cytoplasm; clathrin coated vesicle membrane; coated pit

Molecular Function: protein binding, bridging; protein binding; actin binding; phosphoinositide binding

Biological Process: receptor-mediated endocytosis; actin cytoskeleton organization and biogenesis