Rabbit anti-Human HHIP Polyclonal Antibody | anti-HHIP antibody

HHIP, NT (HHIP, HIP, Hedgehog-interacting protein) (AP)

Cat. Num. AAA6306362

• Gene Names: HHIP; HIP
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HHIP; Polyclonal Antibody; NT (HHIP; HIP; Hedgehog-interacting protein) (AP); Hedgehog-interacting protein; anti-HHIP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-HHIP antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: HHIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-54 amino acids from the N-terminal region of human HHIP.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(HHIP Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the HHIP antibody detected the HHIP protein (arrow).)

Related Product Information for anti-HHIP antibody

This gene encodes a protein similar to the mouse hedgehog-interacting protein, a regulatory component of the hedgehog signalling pathway. Members of the hedgehog family are evolutionarily conserved proteins which are involved in many fundamental processes in embryonic development, including anteroposterior patterns of limbs and regulation of left-right asymmetry.

Product Categories/Family for anti-HHIP antibody

Antibodies; Sonic Hedgehog (SHH)

References

Pillai, S.G., et al. Am. J. Respir. Crit. Care Med. (2010). In press: Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Liu, J.Z., et al. Twin Res Hum Genet 13(2):179-193(2010). Cho, M.H., et al. Nat. Genet. 42(3):200-202(2010). Zhao, J., et al. BMC Med. Genet. 11, 96 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 20143973
• NCBI GeneID: 64399
• NCBI Accession #: NP_071920.1
• NCBI GenBank Nucleotide #: NM_022475.2
• UniProt Accession #: Q96QV1; Q6PK09; Q8NCI7; Q9BXK3; Q9H1J4; Q9H7E7
• Molecular Weight: 78,851 Da
• NCBI Official Full Name: hedgehog-interacting protein
• NCBI Official Synonym Full Names: hedgehog interacting protein
• NCBI Official Symbol: HHIP
• NCBI Official Synonym Symbols: HIP
• NCBI Protein Information: hedgehog-interacting protein
• UniProt Protein Name: Hedgehog-interacting protein
• Protein Family: HHIP-like protein
• UniProt Gene Name: HHIP
• UniProt Synonym Gene Names: HIP; HHIP; HIP
• UniProt Entry Name: HHIP_HUMAN

NCBI Description

This gene encodes a member of the hedgehog-interacting protein (HHIP) family. The hedgehog (HH) proteins are evolutionarily conserved protein, which are important morphogens for a wide range of developmental processes, including anteroposterior patterns of limbs and regulation of left-right asymmetry in embryonic development. Multiple cell-surface receptors are responsible for transducing and/or regulating HH signals. The HHIP encoded by this gene is a highly conserved, vertebrate-specific inhibitor of HH signaling. It interacts with all three HH family members, SHH, IHH and DHH. Two single nucleotide polymorphisms (SNPs) near this gene are significantly associated with risk of chronic obstructive pulmonary disease (COPD). A single nucleotide polymorphism in this gene is also strongly associated with human height.[provided by RefSeq, Feb 2011]

Uniprot Description

HHIP: Modulates hedgehog signaling in several cell types including brain and lung through direct interaction with members of the hedgehog family. Belongs to the HHIP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q28-q32

Cellular Component: cell surface; integral to plasma membrane; cytoplasm; extracellular region

Molecular Function: protein binding; oxidoreductase activity, acting on the CH-OH group of donors, quinone or similar compound as acceptor; zinc ion binding; quinone binding

Biological Process: smoothened signaling pathway; dorsal/ventral pattern formation; skeletal morphogenesis; negative regulation of smoothened signaling pathway; neuroblast proliferation; carbohydrate metabolic process; regulation of fibroblast growth factor receptor signaling pathway; negative regulation of signal transduction