Rabbit anti-Human HEYL Polyclonal Antibody | anti-HEYL antibody

HEYL, NT (HEYL, BHLHB33, HRT3, Hairy/enhancer-of-split related with YRPW motif-like protein, Class B basic helix-loop-helix protein 33, Hairy-related transcription factor 3) (Biotin)

Cat. Num. AAA6306276

• Gene Names: HEYL; HEY3; HRT3; HESR3; bHLHb33
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HEYL; Polyclonal Antibody; NT (HEYL; BHLHB33; HRT3; Hairy/enhancer-of-split related with YRPW motif-like protein; Class B basic helix-loop-helix protein 33; Hairy-related transcription factor 3) (Biotin); Hairy-related transcription factor 3; anti-HEYL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-HEYL antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: HEYL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-54 amino acids from the N-terminal region of human HEYL.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(HEYL Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HEYL antibody detected the HEYL protein (arrow).)

Immunofluorescence (IF)

(Fluorescent confocal image of Hela cell stained with HEYL Antibody (N-term).HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HEYL primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HEYL immunoreactivity is localized to Mitochondria significantly.)

Related Product Information for anti-HEYL antibody

This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The sequence of the encoded protein contains a conserved bHLH and orange domain, but its YRPW motif has diverged from other HESR family members. It is thought to be an effector of Notch signaling and a regulator of cell fate decisions. Alternatively spliced transcript variants have been found, but their biological validity has not been determined. [provided by RefSeq].

Product Categories/Family for anti-HEYL antibody

Antibodies; Transcription Factors

References

Kokubo, H., et al. Trends Cardiovasc. Med. 15(5):190-194(2005). Colland, F., et al. Genome Res. 14(7):1324-1332(2004). Iso, T., et al. J. Cell. Physiol. 194(3):237-255(2003). Nakagawa, O., et al. Proc. Natl. Acad. Sci. U.S.A. 97(25):13655-13660(2000). Firulli, B.A., et al. J. Biol. Chem. 275(43):33567-33573(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 105990530
• NCBI GeneID: 26508
• NCBI Accession #: NP_055386.1
• NCBI GenBank Nucleotide #: NM_014571
• UniProt Accession #: Q9NQ87; Q5TG99
• Molecular Weight: 35,087 Da
• NCBI Official Full Name: Homo sapiens hes-related family bHLH transcription factor with YRPW motif-like (HEYL), mRNA
• NCBI Official Synonym Full Names: hes-related family bHLH transcription factor with YRPW motif-like
• NCBI Official Symbol: HEYL
• NCBI Official Synonym Symbols: HEY3; HRT3; HESR3; bHLHb33
• NCBI Protein Information: hairy/enhancer-of-split related with YRPW motif-like protein; HEY-like protein; HRT-3; class B basic helix-loop-helix protein 33; hHRT3; hHeyL; hairy-related transcription factor 3; hairy/enhancer-of-split related with YRPW motif 3
• UniProt Protein Name: Hairy/enhancer-of-split related with YRPW motif-like protein
• Protein Family: Hairy/enhancer-of-split related with YRPW motif-like protein
• UniProt Gene Name: HEYL
• UniProt Synonym Gene Names: BHLHB33; HRT3; hHeyL; bHLHb33; HRT-3; hHRT3
• UniProt Entry Name: HEYL_HUMAN

NCBI Description

This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The sequence of the encoded protein contains a conserved bHLH and orange domain, but its YRPW motif has diverged from other HESR family members. It is thought to be an effector of Notch signaling and a regulator of cell fate decisions. Alternatively spliced transcript variants have been found, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]