Rabbit anti-Human HDAC2 Polyclonal Antibody | anti-HDAC2 antibody

HDAC2, ID (HDAC2, Histone deacetylase 2) (AP)

Cat. Num. AAA6305889

• Gene Names: HDAC2; HD2; RPD3; YAF1
• Reactivity: Human
• Applications: Immunofluorescence, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HDAC2; Polyclonal Antibody; ID (HDAC2; Histone deacetylase 2) (AP); Histone deacetylase 2; anti-HDAC2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-HDAC2 antibody: Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: HDAC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 417~447 amino acids from the Central region of human HDAC2.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(HDAC2 Antibody (Center) western blot analysis in SH-SY5Y cell line lysates (35ug/lane).This demonstrates the HDAC2 antibody detected the HDAC2 protein (arrow).)

Immunohistochemistry (IHC)

(HDAC2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HDAC2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(HDAC2 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Fluorescent confocal image of Hela cell stained with HDAC2 Antibody (Center).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HDAC2 immunoreactivity is localized to Nucleus significantly.)

Related Product Information for anti-HDAC2 antibody

This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events.

Product Categories/Family for anti-HDAC2 antibody

Antibodies; Enzymes; Deacetylase

References

Ishikawa, F., et al. Oncogene 29(6):909-919(2010). Bush, E.W., et al. Circ. Res. 106(2):272-284(2010). Krishnan, M., et al. Oncogene 29(2):305-312(2010). Lehmann, A., et al. BMC Cancer 9, 395 (2009). Hassig, C.A., et al. Proc. Natl. Acad. Sci. U.S.A. 95(7):3519-3524(1998).

NCBI and Uniprot Product Information

• NCBI GI #: 293336691
• NCBI GeneID: 3066
• NCBI Accession #: NP_001518.3
• NCBI GenBank Nucleotide #: NM_001527.3
• UniProt Accession #: Q92769; Q5SRI8; Q5SZ86; Q8NEH4; B4DL58; E1P561
• Molecular Weight: 55,364 Da
• NCBI Official Full Name: histone deacetylase 2
• NCBI Official Synonym Full Names: histone deacetylase 2
• NCBI Official Symbol: HDAC2
• NCBI Official Synonym Symbols: HD2; RPD3; YAF1
• NCBI Protein Information: histone deacetylase 2; YY1-associated factor 1; transcriptional regulator homolog RPD3
• UniProt Protein Name: Histone deacetylase 2
• UniProt Gene Name: HDAC2
• UniProt Synonym Gene Names: HD2
• UniProt Entry Name: HDAC2_HUMAN

NCBI Description

This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]

Uniprot Description

HDAC2: a transcriptional regulator of the histone deacetylase family, subfamily 1. Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation plays a role in epigenetic repression and transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.

Protein type: Deacetylase; Transcription, coactivator/corepressor; EC 3.5.1.98; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleoplasm; heterochromatin; transcription factor complex; protein complex; Sin3 complex; ESC/E(Z) complex; nuclear chromatin; cytoplasm; replication fork; NuRD complex; nucleus

Molecular Function: deacetylase activity; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; enzyme binding; nucleosomal DNA binding; NAD-dependent histone deacetylase activity (H3-K14 specific); sequence-specific DNA binding; heat shock protein binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); chromatin binding; histone deacetylase activity; transcription factor activity; transcription factor binding

Biological Process: establishment and/or maintenance of chromatin architecture; nerve growth factor receptor signaling pathway; positive regulation of proteolysis; positive regulation of transcription, DNA-dependent; positive regulation of collagen biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; negative regulation of cell cycle; cardiac muscle cell development; negative regulation of gene expression, epigenetic; negative regulation of cardiac muscle cell proliferation; positive regulation of cell proliferation; dendrite development; circadian regulation of gene expression; positive regulation of oligodendrocyte differentiation; response to drug; transcription, DNA-dependent; negative regulation of transcription factor activity; hippocampus development; histone deacetylation; regulation of protein kinase B signaling cascade; response to cocaine; odontogenesis of dentine-containing teeth; chromatin remodeling; ATP-dependent chromatin remodeling; negative regulation of MHC class II biosynthetic process; maintenance of chromatin silencing; epidermal cell differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; gene expression; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; blood coagulation; negative regulation of apoptosis