Rabbit NFkB, p65 Polyclonal Antibody | anti-NKIRAS2 antibody

NFkB, p65 (NF-kB, Nuclear Factor kappa B, RelA)

Cat. Num. AAA630587

• Gene Names: NKIRAS2; KBRAS2; kappaB-Ras2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: NFkB; p65; Polyclonal Antibody; p65 (NF-kB; Nuclear Factor kappa B; RelA); Anti -NFkB; anti-NKIRAS2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes Human V-rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA). Species Crossreactivity: Human, Mouse, Rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.

• Applicable Applications for anti-NKIRAS2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Immunohistochemistry: 5ug/ml; ELISA: 1:1000-1:6000; Western Blot: 1:200-1:2000
• Immunogen: Synthetic peptide, KLH-conjugated. Epitope: Residues surrounding S536 of human p65 (RelA) protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(RELA (5ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human kidney, followed by biotinylated goat rabbit IgG secondary, alkaline phosphatase-streptavidin and chromogen.)

Product Categories/Family for anti-NKIRAS2 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 222136632
• NCBI GeneID: 28511
• NCBI Accession #: NP_001138401.1
• NCBI GenBank Nucleotide #: NM_001144929.1
• UniProt Accession #: Q9NYR9; Q6PK52; Q96KC7; Q9NSX1; A6NCZ5; B3KNN0; B4DNM3
• Molecular Weight: 21,508 Da
• NCBI Official Full Name: NF-kappa-B inhibitor-interacting Ras-like protein 2 isoform c
• NCBI Official Synonym Full Names: NFKB inhibitor interacting Ras-like 2
• NCBI Official Symbol: NKIRAS2
• NCBI Official Synonym Symbols: KBRAS2; kappaB-Ras2
• NCBI Protein Information: NF-kappa-B inhibitor-interacting Ras-like protein 2; kappa B-Ras protein 2; I-kappa-B-interacting Ras-like protein 2; NFKB inhibitor interacting Ras-like protein 2
• UniProt Protein Name: NF-kappa-B inhibitor-interacting Ras-like protein 2
• Protein Family: NF-kappa-B inhibitor-interacting Ras-like protein
• UniProt Gene Name: NKIRAS2
• UniProt Synonym Gene Names: KBRAS2; Kappa B-Ras protein 2; KappaB-Ras2
• UniProt Entry Name: KBRS2_HUMAN

Uniprot Description

NKIRAS2: Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF- kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB. Belongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric, Ras; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: membrane; cytoplasm

Molecular Function: GTPase activity; GTP binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; metabolic process; small GTPase mediated signal transduction