Rabbit anti-Human HBE1v Polyclonal Antibody | anti-HBE1v antibody

HBE1v (HBE1, HBE, Hemoglobin subunit epsilon, Epsilon-globin, Hemoglobin epsilon chain) (AP)

Cat. Num. AAA6305592

• Gene Names: HBE1; HBE
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HBE1v; Polyclonal Antibody; HBE1v (HBE1; HBE; Hemoglobin subunit epsilon; Epsilon-globin; Hemoglobin epsilon chain) (AP); HBE1; Hemoglobin epsilon chain; anti-HBE1v antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HBE1v.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-HBE1v antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 55-83 in the central region of human HBE1v, UniProt Accession #NP_005321.1; P02100.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS6000316. This demonstrates that MBS6000316 detected the HBE1 protein (arrow).)

Product Categories/Family for anti-HBE1v antibody

Antibodies; Hemoglobin

References

1. Nuinoon, M., et al. Hum. Genet. (2009). In press 2. Tachavanich, K., et al. Southeast Asian J. Trop. Med. Public Health 40(2):306-316(2009). 3. Naka, I., et al. Biochem. Genet. 46 (11-12), 708-711 (2008). 4. Winichagoon, P., et al. Transl Res 152(4):178-184(2008). 5. Das, B., et al. Ann. Hum. Biol. 35(4):422-431(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4885393
• NCBI GeneID: 3046
• NCBI Accession #: NP_005321.1
• NCBI GenBank Nucleotide #: NM_005330.3
• UniProt Accession #: P02100; Q6FH44
• Molecular Weight: 16,203 Da
• NCBI Official Full Name: hemoglobin subunit epsilon
• NCBI Official Synonym Full Names: hemoglobin, epsilon 1
• NCBI Official Symbol: HBE1
• NCBI Official Synonym Symbols: HBE
• NCBI Protein Information: hemoglobin subunit epsilon
• UniProt Protein Name: Hemoglobin subunit epsilon
• UniProt Gene Name: HBE1
• UniProt Synonym Gene Names: HBE
• UniProt Entry Name: HBE_HUMAN

NCBI Description

The epsilon globin gene (HBE) is normally expressed in the embryonic yolk sac: two epsilon chains together with two zeta chains (an alpha-like globin) constitute the embryonic hemoglobin Hb Gower I; two epsilon chains together with two alpha chains form the embryonic Hb Gower II. Both of these embryonic hemoglobins are normally supplanted by fetal, and later, adult hemoglobin. The five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5'-epsilon - G-gamma - A-gamma - delta - beta-3' [provided by RefSeq, Jul 2008]

Uniprot Description

HBE1: The epsilon chain is a beta-type chain of early mammalian embryonic hemoglobin. Belongs to the globin family.

Protein type: Carrier

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: hemoglobin complex; cytosol

Molecular Function: iron ion binding; heme binding; hemoglobin alpha binding; oxygen binding; oxygen transporter activity

Biological Process: oxygen transport; protein heterooligomerization; negative regulation of transcription from RNA polymerase II promoter; blood coagulation