Rabbit GTPBP2 Polyclonal Antibody | anti-GTPBP2 antibody

GTPBP2, CT (GTPBP2, GTP-binding protein 2) (APC)

Cat. Num. AAA6304999

• Reactivity: Human, Hamster, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GTPBP2; Polyclonal Antibody; CT (GTPBP2; GTP-binding protein 2) (APC); GTP-binding protein 2; anti-GTPBP2 antibody

• Host: Rabbit
• Reactivity: Human, Hamster, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-GTPBP2 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GTPBP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 543-572 amino acids from the C-terminal region of human GTPBP2.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(GTPBP2 Antibody (C-term) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the GTPBP2 antibody detected the GTPBP2 protein (arrow).)

Western Blot (WB)

(GTPBP2 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the GTPBP2 antibody detected the GTPBP2 protein (arrow).)

Western Blot (WB)

(GTPBP2 Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the GTPBP2 antibody detected the GTPBP2 protein (arrow).)

Immunohistochemistry (IHC)

(GTPBP2 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GTPBP2 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC)

(GTPBP2 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GTPBP2 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-GTPBP2 antibody

GTP-binding proteins, or G proteins, constitute a superfamily capable of binding GTP or GDP. G proteins are activated by binding GTP and are inactivated by hydrolyzing GTP to GDP. This general mechanism enables G proteins to perform a wide range of biologic activities.

Product Categories/Family for anti-GTPBP2 antibody

Antibodies; Binding Proteins

References

Mungall, A.J., et al. Nature 425(6960):805-811(2003). Watanabe, M., et al. Gene 256 (1-2), 51-58 (2000): Kudo, H., et al. Biochem. Biophys. Res. Commun. 272(2):456-465(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 45593140
• NCBI GeneID: 54676
• NCBI Accession #: NP_061969.3
• NCBI GenBank Nucleotide #: NM_019096.3
• UniProt Accession #: Q9BX10; Q5T7E8; Q8ND84; Q8TAH7; Q8WUA5; Q9HCS9; Q9NRU4; Q9NX60
• Molecular Weight: 65,768 Da
• NCBI Official Full Name: GTP-binding protein 2
• NCBI Official Synonym Full Names: GTP binding protein 2
• NCBI Official Symbol: GTPBP2
• NCBI Protein Information: GTP-binding protein 2
• UniProt Protein Name: GTP-binding protein 2
• Protein Family: GTP-binding protein
• UniProt Gene Name: GTPBP2
• UniProt Entry Name: GTPB2_HUMAN

NCBI Description

GTP-binding proteins, or G proteins, constitute a superfamily capable of binding GTP or GDP. G proteins are activated by binding GTP and are inactivated by hydrolyzing GTP to GDP. This general mechanism enables G proteins to perform a wide range of biologic activities.[supplied by OMIM, Jan 2003]

Uniprot Description

GTPBP2: Belongs to the GTPBP1 GTP-binding protein family. Up-regulated by IFNG/IFN-gamma in human monocytic cell line THP-1. 4 isoforms of the human protein are produced by alternative splicing

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: intracellular membrane-bound organelle

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: metabolic process