Rabbit anti-Rat Akt1, phosphorylated (Ser472) Polyclonal Antibody | anti-AKT1 antibody
Anti-Akt1, phosphorylated (Ser472) (Rac PKa, PKBa)
Gene Names
AKT1; AKT; PKB; RAC; PRKBA; MGC99656; PKB-ALPHA; RAC-ALPHA
Reactivity
Rat
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
Akt1; phosphorylated (Ser472); Polyclonal Antibody; Anti-Akt1; phosphorylated (Ser472) (Rac PKa; PKBa); Rac PKa; PKBa; anti-AKT1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The rat phospho-peptide is 93% homologous in Mouse/ human/ chicken and bovine.
Purity/Purification
Serum
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
Applicable Applications for anti-AKT1 antibody
ELISA; Western Blot
Application Notes
Recommended Dilutions: Western Blot: 1:1000-5K000
ELISA (1:10,000-1:100,000; using 50-100 ng of control peptide/well).
ELISA (1:10,000-1:100,000; using 50-100 ng of control peptide/well).
Immunogen
Rat AKT1 peptide (~C-terminus, 15 aa) containing a phosphorylated Ser472, coupled to KLH
Source Information
Rat
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Temperature: Blue Ice
Shipping Temperature: Blue Ice
Related Product Information for anti-AKT1 antibody
Putative human homolog of the proto-oncogene v-akt of the acutely transforming retrovirus AKT8 have been cloned. These protein-serine/threonine kinase proteins have a catalytic domain closely related to both PKA and PKC and have been designated rac (related to A and C kinases), pkb (Protein kinase B) or Akt.
RAC protein kinase family members feature pleckstrin homology (PH) domain at the amino terminus and a protein-serine/threonine kinase catalytic domain at the carboxy terminus. The Amino terminal domain (referred to as AH-Akt Homology domain) spans from 1-148 amino acids and contains the PH domain, a region found in diverse group of signaling proteins. The PH domain (amino acids 1-106) has been implicated in interactions with other proteins such as G-protein bg subunits, as well as phosphoinositides. The kinase domain is located between residues 148 to 411. These enzymes are activated by diverse ligands such as PGDF, EGF and basic FG in NIH 3T3, Rat-1 or Swiss-3T3 cells.
AKT1 (RAC-PK-a or PKB-a) is the human homolog of v-akt and is identical to RAC gene. The protein has been observed to show different migratory patterns on a western blot according to the state of phosphorylation of the protein. Phosphatase treatment has been shown to result in inactivation of the protein.
RAC protein kinase family members feature pleckstrin homology (PH) domain at the amino terminus and a protein-serine/threonine kinase catalytic domain at the carboxy terminus. The Amino terminal domain (referred to as AH-Akt Homology domain) spans from 1-148 amino acids and contains the PH domain, a region found in diverse group of signaling proteins. The PH domain (amino acids 1-106) has been implicated in interactions with other proteins such as G-protein bg subunits, as well as phosphoinositides. The kinase domain is located between residues 148 to 411. These enzymes are activated by diverse ligands such as PGDF, EGF and basic FG in NIH 3T3, Rat-1 or Swiss-3T3 cells.
AKT1 (RAC-PK-a or PKB-a) is the human homolog of v-akt and is identical to RAC gene. The protein has been observed to show different migratory patterns on a western blot according to the state of phosphorylation of the protein. Phosphatase treatment has been shown to result in inactivation of the protein.
Product Categories/Family for anti-AKT1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
55,686 Da
NCBI Official Full Name
AKT1
NCBI Official Synonym Full Names
v-akt murine thymoma viral oncogene homolog 1
NCBI Official Symbol
AKT1
NCBI Official Synonym Symbols
AKT; PKB; RAC; PRKBA; MGC99656; PKB-ALPHA; RAC-ALPHA
NCBI Protein Information
RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; protein kinase B; proto-oncogene c-Akt; rac protein kinase alpha
UniProt Protein Name
RAC-alpha serine/threonine-protein kinase
Protein Family
UniProt Gene Name
AKT1
UniProt Synonym Gene Names
PKB; RAC
UniProt Entry Name
AKT1_HUMAN
Similar Products
Product Notes
The AKT1 akt1 (Catalog #AAA630483) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Akt1, phosphorylated (Ser472) (Rac PKa, PKBa) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Akt1, phosphorylated (Ser472) can be used in a range of immunoassay formats including, but not limited to, ELISA; Western Blot. Recommended Dilutions: Western Blot: 1:1000-5K000 ELISA (1:10,000-1:100,000; using 50-100 ng of control peptide/well). Researchers should empirically determine the suitability of the AKT1 akt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Akt1, phosphorylated (Ser472), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
