Rabbit anti-Human GTF2A1L Polyclonal Antibody | anti-GTF2A1L antibody

GTF2A1L, CT (GTF2A1L, ALF, GTF2A1LF, TFIIA-alpha and beta-like factor, General transcription factor II A, 1-like factor) (AP)

Cat. Num. AAA6304811

• Gene Names: GTF2A1L; ALF
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GTF2A1L; Polyclonal Antibody; CT (GTF2A1L; ALF; GTF2A1LF; TFIIA-alpha and beta-like factor; General transcription factor II A; 1-like factor) (AP); 1-like factor; anti-GTF2A1L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-GTF2A1L antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GTF2A1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 398-428 amino acids from the C-terminal region of human GTF2A1L.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(GTF2A1L Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GTF2A1L antibody detected the GTF2A1L protein (arrow).)

Related Product Information for anti-GTF2A1L antibody

The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involve the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology. Alternative splicing for this locus has been observed and two variants, encoding distinct isoforms, have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

Product Categories/Family for anti-GTF2A1L antibody

Antibodies; Transcription Factors

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Kim, M., et al. J. Biol. Chem. 281(45):34288-34298(2006). Howe, M.L., et al. Stem Cells Dev. 15(2):175-190(2006). Huang, M., et al. Int. J. Mol. Med. 17(4):599-604(2006). Upadhyaya, A.B., et al. J. Biol. Chem. 277(37):34208-34216(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 26787967
• NCBI GeneID: 11036
• NCBI Accession #: NP_006863.2
• NCBI GenBank Nucleotide #: NM_006872.2
• UniProt Accession #: Q9UNN4; Q53FD9; Q5D050; B4DY14
• Molecular Weight: 48,504 Da
• NCBI Official Full Name: Homo sapiens general transcription factor IIA, 1-like (GTF2A1L), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: general transcription factor IIA, 1-like
• NCBI Official Symbol: GTF2A1L
• NCBI Official Synonym Symbols: ALF
• NCBI Protein Information: TFIIA-alpha and beta-like factor; GTF2A1-like factor; TFIIA large subunit isoform ALF; TFIIA-alpha/beta-like factor; general transcription factor II A, 1-like factor
• UniProt Protein Name: TFIIA-alpha and beta-like factor
• Protein Family: TFIIA-alpha and beta-like factor
• UniProt Gene Name: GTF2A1L
• UniProt Synonym Gene Names: ALF; GTF2A1LF
• UniProt Entry Name: TF2AY_HUMAN

NCBI Description

The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involve the effects of transcription factor IIA (TFIIA) on the interaction between TATA-binding protein (TBP) and DNA. This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology. Alternative splicing for this locus has been observed and two variants, encoding distinct isoforms, have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Mar 2014]